News

The BIOTECnico PhD program is committed to organize different activities for its PhD students.

Click on the following link to scroll all the events associated with BIOTECnico: http://www.scoop.it/t/ibb/?&tag=BIOTECnico

 

BIOTECnico Seminars

BIOTECnico is organizing a PhD Program seminar cycle, where alumni and last year students will be invited to share their PhD outcomes.

First seminar will be January 19, 2021 at 4 pm. Save the date.

 

David Botequim to Defend PhD Thesis in Biotechnology and Biosciences

David Botequim defended his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, onthe 18th of November 2020, at 10:30 am. During the last years, and under the supervision of Pedro Paulo from CQE and Miguel Prazeres from BERG-iBB, David studied fluorescently-labeled DNA probes conjugated onto gold nanoparticles for the improved detection of nucleic acids. The title of his thesis is “Fluorescently-labeled DNA Probes Functionalized onto Gold Nanorods for Signal Enhancement in Nucleic Acids’ Detection”.

 

André Nascimento Defends PhD Thesis in Biotechnology and Biosciences

André Nascimento defended his PhD thesis in Biotechnology and Biosciences on Monday the 6th april 2020 (14:00 H). During the last years, and under the supervision of Ana Azevedo from BERG-iBB and Steven Cramer and Pankaj Karande from the Rensselaer Polytechnic Institute (US), André developed and explored affinity peptides and multimodal ligands for the purification of Fab antibody fragments. The title of the thesis is “Development of a novel purification platform for Fab antibody fragments based on affinity peptides and multimodal ligands”.

 

Amir Hassan defends PhD Thesis in Biotechnology and Biosciences

Amir Hassan defended his PhD thesis in Biotechnology and Biosciences on the 28th january 2020 (14:30 H, amphitheater PA-3, Mathematics Building). During the last years, and under the supervision of Isabel Sá-Correia from BSRG-iBB, Amir studied the role of the lipopolysaccharide O-antigen on the adaptation of the Bhurkolderia cepacia complex to the cystic fibrosis environment. The title of the thesis is “Adaptation of Bhurkolderia cepacia complex bacteria to the cystic fibrosis lung: focus on lipopolysaccharide O-antigen and cel wall physical properties”.

 

Nuno Bourbon delivers oral presentation at Microbiotec’19

Nuno Bourbon, PhD student from the BIOTECnico doctoral programme at iBB-BSRG, presented his work during the symposium on “Industrial and Food Microbiology” of the Congress of Microbiology and Biotechnology 2019 (Microbiotec’19), which was held between the 5th and 7th of December 2019 in Coimbra, Portugal. The presentation, entitled “Use of Hanseniaspora guilliermondii and Hanseniaspora opuntiae in co-fermentation with Saccharomyces cerevisiae to enhance the aromatic profile of craft beer”, focused on the potential of using two distinct Hanseniaspora yeast species to improve the organoleptic properties of beer.

 

Pedro Pais defends his PhD thesis in Biotechnology and Biosciences

Pedro Pais defended his PhD thesis in Biotechnology and Biosciences at Instituto Superior Técnico, on the 27th september 2019 (10:00 H, room PA-3). During the last years, and under the supervision of Miguel Teixeira from BSRG-iBB, Pedro studied the intricacies of the regulatory network that controls drug resistance in the fungus Candida glabrata. The title of his thesis is “Unveiling the complex regulatory network governing antifungal drug resistance in Candida glabrata: from new regulators to new effectors”.

 

André Nascimento, Claudia Alves, Ana Rita Santos and Carlos Rodrigues participate in ESBES 2018

The 12th Symposium of the European Society of Biochemical Sciences (ESBES 2018) was held in Lisboa, Portugal on September 9-12 2018. André Nascimento gave an oral communication entitled “Phage display-aided identification of affinity peptide ligands against Kappa Fab fragments” (A. Nascimento, A. Mullerpatan, M.R. Aires-Barros, P. Karande, A.M. Azevedo, S. Cramer).

Claudia Alves, Ana Rita Santos and Carlos Rodrigues were also present and each presented a poster entitled, respectively, “Improving minicircle DNA production by in silico re-design of the ParA resolvase untranslated region”, “Multimodal chromatography of supercoiled minicircles: a closer look into DNA-ligand interactions”  and “Searching for transaminase activity in bacteria from the Azores”.

 

Students present at the 3rd Joint Workshop

The 3rd joint workshop of the BIOTECnico and AEM FCT PhD programs was held on June 11-12 2018, in Lisbon at IST.

•  The BIOTECnico students from class 2015 have presented their main results in a 15 min oral communication:

Amir Hassan: “Burkholderia cepacia complex bacteria surface variation during long-term infection of the cystic fibrosis lung”

Pedro Pais: “Exploring the transcriptional landscape of antifungal resistance in Candida glabrata: from new regulators to new effectors”

André Nascimento: “Evolution of phage biopanning strategies to identify affinity peptides to purify Fab fragments.”

David Botequim: “Gold nano-beacons for nucleic acid detection: biosensing with plasmon-enhanced fluorescence”

•  The BIOTECnico students from class 2016 have presented their main results in a 10 min oral communication:

Mafalda Cavalheiro: “From susceptible to multiple azole resistant: the in vitro evolution of a Candida glabrata clinical isolate”

Cláudia Alves: “Escherichia coli genome engineering for improved minicircle production”

Andreia Pimenta: “Differential gene expression of Burkholderia cenocepacia trimeric autotransporter adhesin BCAM2418 in the early stages of bacteria-host-cell interactions”

Rui Silva: “Determination of proteolytic activity in real-time using plasmonic nanosensors”

 

•  The BIOTECnico students from class 2017 also presented their main results in a 10 min oral communication:

Carlos Rodrigues: “Improving transaminase activity for industrial bioprocess applications”

Ana Rita Silva: “Biomanufacturing of DNA-origami nanostructures for bio-imaging”

Sara Salazar: “Mechanistic insights on the role of CgHaa1- and CgPdr1- pathways in stress residence and pathogenesis of Candida glabrata

Tiago Pita: “Deciphering the Burkholderia cepacia complex virulome using the infection model Caenorhabditis elegans

 

André Nascimento publishes article in Separation and Purification Technology

A research article entitled “Studies on the purification of antibody fragments” by A. Nascimento, I.F.Pinto, V. Chu, M.R. Aires-Barros, J.P. Conde and A.M.Azevedo was published in Separation and Purification Technology, 195: 388-397, 2018. To know more follow this link.

 

Students present at the 2nd Joint Workshop

The 2nd joint workshop of the BIOTECnico and AEM FCT PhD programs was held on February 15-16 2017, in Lisbon at IST. The BIOTECnico students from class 2015 have presented their main results to the advisory board.

Amir Hassan: “Burkholderia cepacia complex bacteria variation during long-term infection of the cystic fibrosis lung: focus on the LPS O-antigen”

André Nascimento: ““Evaluating conventional preparation methods of antibody fragments and rapid screening of alternative binding conditions using a microfluidic-based approach”

David Botequim: “Plasmonic biosensing using biotin functionalized gold nanorods”

Pedro Pais: “Transcription regulatory networks controlling antifungal drug resistance in Candida glabrata

 

Students from class 2016 also presented the main topic of their PhD program toghther with some preliminary results.

Andreia Pimenta: “Functional characterization of virulence-associated Trimeric Autotransporter Adhesins from the human pathogen Burkholderia cenocepacia” -

Cláudia Alves: “CRISPR/Cas9-based engineering of Escherichia coli for biobrick-based minicircles”

Rui Silva: “Biotechnological applications of carbon-dots: shine bright like a diamond”

Mafalda Cavalheiro: “In the crossroad between drug resistance and virulence in C. glabrata

 

Pedro Pais publishes article in Frontiers in Microbiology

A research article entitled “Membrane proteomics analysis of the Candida glabrata response to 5-flucytosine: unveiling the role and regulation of the drug efflux transporters CgFlr1 and CgFlr2” by P. Pais, C. Pires, C. Costa, M. Okamoto, H. Chibana and M.C. Teixeira was published in Frontiers in Microbiology, 7:2045, 2016. To know more follow this link.

 

Amir Hassan participates in the XIX National Congress of Biochemistry

The XIX National Congress of Biochemistry was held on December 8–10, 2016, in Guimarães, Portugal. Amir Hassan presented a poster entitled “Lipopolysaccharide O-antigen modification during long-term infection in cystic fibrosis: comparative genomic analysis of 11 Burkholderia cenocepacia sequential clonal variants” by A.A. Hassan, S.C. dos Santos, R.F. Maldonado, C.P. Coutinho, F. Di Lorenzo, R. Sebra, M. Dillon, V.S. Cooper, A. Molinaro, M. Valvano and I. Sá-Correia.

 

Pedro Pais publishes article in Frontiers in Cellular and Infection Microbiology

A research article entitled “Transcriptional control of drug resistance, virulence and immune system evasion in pathogenic fungi: a cross-species comparison” by P. Pais, C. Costa, M. Cavalheiro, D. Romão and M.C. Teixeira was published in Frontiers in Cellular and Infection Microbiology, 6: 131, 2016. To know more follow this link.

 

Pedro Pais publishes article in Molecular and Cellular Proteomics

A research article entitled “Membrane proteomewide response to the antifungal drug clotrimazole in Candida glabrata: role of the transcription factor CgPdr1 and the Drug:H+ Antiporters CgTpo1_1 and CgTpo1_2″ by P. Pais, C. Costa, C. Pires, K. Shimizu, H. Chibana and M.C. Teixeira was published in Molecular and Cellular Proteomics, 15(1):57-72, 2016. To know more follow this link.

 

Pedro Pais and Maflada Cavalheiro participate in PYFF 6

The 6th Conference on Physiology of Yeast and Filamentous Fungi -PYFF 6, was held in Lisboa, Portugal. Pedro Pais and Mafalda Cavalheiro were both present and each presented a poster entitled,respectively, “Membrane proteomics approaches unveil new mechanisms of 5-flucytosine and clotrimazole antifungal resistance in the human pathogen Candida glabrata” (P. Pais, C. Costa, C. Pires, D. Romão, M. Cavalheiro, M.C. Teixeira) and “Unveiling the mechanisms of azole drug resistance acquisition in a clinical isolate: a ranscriptomics approach” (M. Cavalheiro, C. Costa, C. Wang, I.M. Miranda, A. Silva-Dias, A.G. Rodrigues, G. Butler, M.C. Teixeira).

 

Students present at the 1st Joint Workshop

The 1st joint workshop of the BIOTECnico and AEM FCT PhD programs was held on January 21-22 2016, in Lisboa at IST. The BIOTECnico students from class 2015 presented the main topic of their PhD program toghther with some preliminary results.

Amir Hassan: “Bacterial adaptive evolution during chronic infection: the case study of the Burkholderia cepacia complex in the cystic fibrosis lung”

André Nascimento:  “Development of a novel purification platform for Fab antibody fragments based on affinity peptide ligands”

David Botequim: “Nano-Beacons: Biosensors with Plasmonic Enhancement of Fluorescence Signalling for Nucleic Acid Detection”

Pedro Pais:  “Antifungal drug resistance in Candida glabrata: the regulators and the effectors”.

 

André Nascimento participates in the XVI COLACRO

The 16th Latin-American Congress on Chromatography – XVI COLACRO – was held on January 5-6, 2016 at the Sciences Faculty of the University of  Lisbon. André Nascimento presented a poster entitled “Convective Flow Devices for the Polishing of Monoclonal Antibodie” by A. Nascimento, S.A.S.L. Rosa, M. Mateus and A.M. Azevedo.

 

Students participate in the MicroBiotec 2015

From 10-12 December 2015, the University of Évora hosted the 6th joint congress of Microbiology and Biotechnology: Microbiotec15, organized by the Portuguese Society of Microbiology and the Portuguese Society of Biotechnology.

microbiotec15

Find the Fellows!

Oral Communication

David Botequim: “Functionalized gold nanoparticles for plasmonic biosensing of nucleic acids” by D. Botequim, A. Jóskowiak, S.A.M. Martins, D.M.F. Prazeres.
Costa, S.M.B., Paulo, P.M.R.,

 

Poster Communication

Amir Hassan: “Variation of O-antigen presence in Burkholderia cepacia complex bacteria lipopolysaccharide (LPS) during chronic lung infection: a 20-year retrospective study at a national cystic fibrosis (CF) center” by A.A. Hassan, C.P. Coutinho, R.F. Maldonado and I. Sá-Correia.

André Nascimento: ” Polishing of Monoclonal Antibodies Streams through Convection Flow Devices” by A. Nascimento, S.A.S.L. Rosa, M. Mateus, A.M. Azevedo.

Pedro Pais: “New mechanisms of 5- flucytosine resistance in the human pathogen Candida glabrata: unveiled throught chemogenomics and proteomics approaches” by P. Pais, C. Costa, C. Pires, R. Santos, M. Cavalheiro, M.C. Teixeira.

News
BIOTECnico Seminar Cycle stating in Jan 2021.
Keywords
Molecular and Cellular Biology | Bioprocess and Biosystems Engineering | Functional and Comparative Genomics | Upstream and Downstream Processing | Computational and Systems Biology | Biosensors and Nanotechnology | Synthetic Biology | Molecular Bioengineering