Cell envelope remodeling in response to stress in yeasts: mechanistic insights and applications
• Isabel Sá-Correia, Professor @ IST/ULisboa, PT
In recent years, several Omics studies allowed a more comprehensive coverage and understanding of the global mechanisms involved in Saccharomyces cerevisiae responses and tolerance to environmental stresses. Among them is the alteration of the composition, physical properties and biological function of yeast plasma membrane and cell wall. This project seeks to provide new insights into the molecular mechanisms behind yeast cell envelope remodelling in the presence of stresses of biotechnological relevance, including acetic acid stress. Acetic acid is a major inhibitory compound widely used as a preservative in the food and beverage industries and its presence in lignocellulosic hydrolysates limits their use in sustainable biorefinery processes.
A better understanding of stress adaptation and tolerance mechanisms in S cerevisiae, at the level of the cell envelope,paves the way to this knowledge extension to non-conventional yeast species. This thesis project also seeks to extend the knowledge gathered for S cerevisiae to non-conventional yeast species that are currently gaining momentum for guiding the design and engineering of more robust cell factories.
Bourbon-Melo, N., Palma, M., Rocha, M. P., Ferreira, A., Bronze, M. R., Elias, H., Sá-Correia, I., Use of Hanseniaspora guilliermondii and Hanseniaspora opuntiae to enhance the aromatic profile of beer in mixed-culture fermentation with Saccharomyces cerevisiae. Food Microbiology, 103678, 2020. DOI: 10.1016/j.fm.2020.103678
Monteiro, P. T., Oliveira, J., Pais, P., Antunes, M., Palma, M., Cavalheiro, M., Galocha, M., Godinho, C. P., Martins., L. C., Bourbon, N., Mota, M. N., Ribeiro, R. A., Viana, R., Sá-Correia, I., Teixeira, M. C., YEASTRACT+: a portal for cross-species comparative genomics of transcription regulation in yeasts. Nucleic Acids Research, 48(D1), D642-D649, 2020.
Bourbon-Melo, N., Palma, M., Rocha, M. P., Ferreira, A., Bronze, M. R., Elias, H., Sá-Correia, I., Use of Hanseniaspora guilliermondii and Hanseniaspora opuntiae in co-fermentation with Saccharomyces cerevisiae to enhance the aromatic profile of craft beer. Microbiotec’19, 5-7 December, 2019, Coimbra, Portugal
DOCTORAL PROGRAM (36 ECTS)
• General Doctoral Training (6 ECTS)
• Advanced Experimental Techniques and methodologies (6 ECTS)
• Bioentrepreneurship (6 ECTS)
• Outreach and Teaching Skills (6 ECTS)
• Molecular and Cellular Biophysics (6 ECTS)
• Microbiomes and Metagenomics (6 ECTS)