Carlos Rodrigues

Class 2017phdCJR

 

PhD thesis

Developing bioprocesses with marine bacteria for sustainable industrial applications

 

Supervisor

Carla C.C.R. Carvalho, Professor @ IST/ULisboa, PT

 

Proposal summary

The microbial diversity that the Ocean offers is tremendous for potential industrial application of biocatalysts. The harsh and broad environment that marine microorganisms face has contributed to the evolution of microbial enzymes that ensure their survival. Microbial enzymes are used in a variety of industries contributing for sustainable processes, but high performance and promiscuous enzymes are still needed. To access new marine microbial enzymes, it is critical the cultivation of bacteria in the laboratory. Current techniques are thought to enable the growth of only ca. 1% of bacteria present in the marine samples. Therefore, it is urgent to develop new cultivation techniques to mimic the natural conditions. This proposal aims to develop novel integrative bioprocesses by accessing the enzymatic machinery of marine bacteria and improving cultivation techniques of ‘unculturable’ bacteria. This will contribute to sustainable industrial applications of marine enzymes with benefits for public health and the environment.

 

PUBLICATIONS

Papers

Rodrigues C. J., de Carvalho C. C. C. R., Phenotypic adaptations help Rhodococcus erythropolis cells during the degradation of paraffin wax. Biotechnology Journal, 1800598, 2019.

Rodrigues CJC, Sanches JM, de Carvalho CCCR, Determining transaminase activity in bacterial libraries by time-lapse imaging. Chemical Communications, 55(90):13538-13541, 2019.

 

Oral Communications

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Marine bacteria from Azores as potential source of transaminases for drug development”, MicroBiotec2017 December 7-8, 2017, Porto, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Degradation of paraffin wax by Rhodococcus erythropolis. 12th ESBES – European Symposium on Biochemical Engineering Sciences, September 2018, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Culturomics approach for the isolation of halophilic strains”, 12th International Congress of Extremophiles, September 16-20, 2018, Ischia, Italy.

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Improving bioprocesses by using adaptation strategies of Rhodococcus”, Biotechnology Virtual 2020, November 16-17, 2020.

 

Poster Communications

Rodrigues, C.J.C., “New marine biocatalysts for sustainable industrial bioprocesses”, PhD open days 2018Instituto Superior Técnico, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Searching for transaminase activity in bacteria from the Azores” 12th ESBES – European Symposium on Biochemical Engineering Sciences, September 2018, Lisboa, Portugal

de Carvalho,C.C.C.R., Rodrigues, C.J.C., Carvalho, F., Pereira, R.F.S., Cabral, J.M.S., Fernandes, P., “Culturomics approaches for finding marine biocatalysts. 12th ESBES – European Symposium on Biochemical Engineering Sciences, September 2018, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Culturomics to Find New Marine Biocatalysts for Industrial Processes”, PhD open days 2019Instituto Superior Técnico, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Trifluoromethylamine production using a transaminase”, Amine biocatalysis 4.0., February 17-19, 2020, Stuttgart, Germany

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Exploring marine environments for new biocatalysts”, PhD open days 2020October 26-28, 2020, Instituto Superior Técnico, Lisboa, Portugal

 

DOCTORAL PROGRAM (36 ECTS)

• General Doctoral Training (6 ECTS)

• Advanced Experimental Techniques and methodologies (6 ECTS)

Class 2017

 

PhD thesis

Developing bioprocesses with marine bacteria for sustainable industrial applications

 

Supervisor

Carla C.R. Carvalho, Professor @ IST/ULisboa, PT

 

Proposal summary

The microbial diversity that the Ocean offers is tremendous for potential industrial application of biocatalysts. The harsh and broad environment that marine microorganisms face has contributed to the evolution of microbial enzymes that ensure their survival. Microbial enzymes are used in a variety of industries contributing for sustainable processes, but high performance and promiscuous enzymes are still needed. To access new marine microbial enzymes, it is critical the cultivation of bacteria in the laboratory. Current techniques are thought to enable the growth of only ca. 1% of bacteria present in the marine samples. Therefore, it is urgent to develop new cultivation techniques to mimic the natural conditions. This proposal aims to develop novel integrative bioprocesses by accessing the enzymatic machinery of marine bacteria and improving cultivation techniques of ‘unculturable’ bacteria. This will contribute to sustainable industrial applications of marine enzymes with benefits for public health and the environment.

 

PUBLICATIONS

Papers

Rodrigues C. J., de Carvalho C. C. C. R., Phenotypic adaptations help Rhodococcus erythropolis cells during the degradation of paraffin wax. Biotechnology Journal, 1800598, 2019.

Rodrigues CJC, Sanches JM, de Carvalho CCCR, Determining transaminase activity in bacterial libraries by time-lapse imaging. Chemical Communications, 55(90):13538-13541, 2019.

 

Oral Communications

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Marine bacteria from Azores as potential source of transaminases for drug development”, MicroBiotec2017 December 7-8, 2017, Porto, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Degradation of paraffin wax by Rhodococcus erythropolis. 12th ESBES – European Symposium on Biochemical Engineering Sciences, September 2018, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Culturomics approach for the isolation of halophilic strains”, 12th International Congress of Extremophiles, September 16-20, 2018, Ischia, Italy.

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Improving bioprocesses by using adaptation strategies of Rhodococcus”, Biotechnology Virtual 2020, November 16-17, 2020.

 

Poster Communications

Rodrigues, C.J.C., “New marine biocatalysts for sustainable industrial bioprocesses”, PhD open days 2018Instituto Superior Técnico, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Searching for transaminase activity in bacteria from the Azores” 12th ESBES – European Symposium on Biochemical Engineering Sciences, September 2018, Lisboa, Portugal

de Carvalho,C.C.C.R., Rodrigues, C.J.C., Carvalho, F., Pereira, R.F.S., Cabral, J.M.S., Fernandes, P., “Culturomics approaches for finding marine biocatalysts. 12th ESBES – European Symposium on Biochemical Engineering Sciences, September 2018, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Culturomics to Find New Marine Biocatalysts for Industrial Processes”, PhD open days 2019Instituto Superior Técnico, Lisboa, Portugal

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Trifluoromethylamine production using a transaminase”, Amine biocatalysis 4.0., February 17-19, 2020, Stuttgart, Germany

Rodrigues, C.J.C., de Carvalho, C.C.C.R., “Exploring marine environments for new biocatalysts”, PhD open days 2020October 26-28, 2020, Instituto Superior Técnico, Lisboa, Portugal

 

DOCTORAL PROGRAM (36 ECTS)

• General Doctoral Training (6 ECTS)

• Advanced Experimental Techniques and methodologies (6 ECTS)

• Bioentrepreneurship (6 ECTS)

• Outreach and Teaching Skills (6 ECTS)

• Chemical and Biological Reaction Engineering (6 ECTS)

• Sensors, Instrumentation and Measurement in Biological Systems (6 ECTS)