Miguel Antunes

Class 2018Miguel Antunes

 

PhD thesis

Gene regulatory networks that control environmental responses in yeasts

 

Supervisors

• Isabel Sá-Correia,Professor @ IST/ULisboa, PT

Collaborators

• Pedro T. Monteiro, Professor @ IST/ULisboa, PT

 

Proposal summary

Most of the cellular processes in yeast are regulated through protein phosphorylation. Protein kinases and phosphatases exert a key role in these regulatory pathways through the control of the phosphorylation state of the associated proteins. A throughout understanding of the regulation of cellular processes by kinases is a complex task as it involves, at least, the identification of the involved kinases activation mechanisms as well as the respective phosphorylation targets during a specific environmental condition. The Hrk1 kinase from S. cerevisiae belonging to the Npr1/Hal5 family of kinases has been associated with the regulation of plasma membrane proteins and shown to be an acetic acid tolerance determinant. However, the underlying signaling pathways and regulatory mechanisms of this kinase – as for most of Npr1/Hal5 kinases – remain poorly characterized. This thesis focus on the integration of Hrk1 into signaling pathways and understanding of its biological role in S. cerevisiae and biotechnologically relevant non-conventional yeasts as it is paramount to guide the design of more robust cell factories with a greater productivity control of high-value metabolites and improved stress resistance.


PUBLICATIONS

Papers

Monteiro, P. T., Oliveira, J., Pais, P., Antunes, M., Palma, M., Cavalheiro, M., Galocha, M., Godinho, C. P., Martins., L. C., Bourbon, N., Mota, M. N., Ribeiro, R. A., Viana, R., Sá-Correia, I., Teixeira, M. C., YEASTRACT+: a portal for cross-species comparative genomics of transcription regulation in yeasts. Nucleic Acids Research48(D1), D642-D649, 2020.

Antunes, M., Palma, M., & Sá-Correia, I., Transcriptional profiling of Zygosaccharomyces bailii early response to acetic acid or copper stress mediated by ZbHaa1. Scientific Reports8(1), 1-14, 2018.

Teixeira, M. C., Monteiro, P. T., Palma, M., Costa, C., Godinho, C. P., Pais, P., Carvalheiro, M., Antunes, M., Lemos, A., Pedreira, T., & Sá-Correia, I. YEASTRACT: an upgraded database for the analysis of transcription regulatory networks in Saccharomyces cerevisiae. Nucleic acids research46(D1), D348-D353, 2018.

 

Poster Communications

Antunes, M., Palma, M., & Sá-Correia, I., “The Protein Kinase Hrk1 Enhances Glucose Uptake Through the Hexose Transporter Hxt1 in Saccharomyces cerevisiae.” 29th International Conference on Yeast Genetics and Molecular Biology (ICYGMB) August 18-22, 2019, Gothenburg, Sweden.

Antunes, M., Oliveira, J., Pais, P., Palma, M., Cavalheiro, M., Galocha, M., Godinho, C. P., Martins., L. C., Bourbon, N., Mota, M. N., Ribeiro, R. A., Viana, R., Monteiro, P. T., Sá-Correia, I., Teixeira, M. C., “YEASTRACT+: a portal for cross-species comparative genomics of transcription regulation in yeasts.” Microbiotec’19 December 5-7, 2019, Coimbra, Portugal.

 

DOCTORAL PROGRAM (36 ECTS)

• General Doctoral Training (6 ECTS)

• Advanced Experimental Techniques (6 ECTS)

• Bioentrepreneurship (6 ECTS)

• Genomics Proteomics and Bioinformatics (6 ECTS)

• Outreach and Teaching Skills (6 ECTS)

• Molecular and Cell Biophysics (6 ECTS)

Advanced courses:

• BioData.pt Crash Course: Microbiome Visualization with Biome-Shiny
• Ready For BioData Management? – Introduction to Data Management Plans
• Elixir PT/BioData.pt – Data Carpentry Workshop – Genomics Data