Sara Salazar

Class 2017Sara Salazar

 

PhD thesis

Mechanistic insights on the role of CgHaa1- and CgPdr1- pathways in stress resilience and pathogenesis of Candida glabrata

 

Supervisor

• Nuno P. Mira , Professor @ IST/ULisboa, PT

Collaborators

• Frederic Devaux, Professor @ Université Pierre et Marie Curie, FR

• Paula Sampaio, Professor @ Universidade do Minho, PT

• Joana Azeredo, Professor @ Universidade do Minho, PT

 

Proposal summary

To successfully thrive in the genitourinary tract C. glabrata has to cope with numerous stresses including the presence of organic acids at a low pH that are produced by the co-colonizing microbiota and also azoles which are used as antifungal agents. This thesis will be focused on elucidating, at a genome-wide scale, how C. glabrata cells respond to these two stresses, either alone or in combination. Emphasis will be put on the role played by the CgPdr1 and CgHaa1 regulons, considering their demonstrated essential role in response to those stresses. It will also be examined whether these two regulatory systems may contribute for pathogenic traits of C. glabrata including virulence in different infection models or ability to form biofilms in biotic or abiotic surfaces.

 

PUBLICATIONS

Papers

Salazar, S.B., Simões, R.S., Pedro, N.A., Pinheiro, M.J., Carvalho, N.F.N.N., Mira, N.P., An overview on conventional and non-conventional therapeutic approaches for the treatment of candidiasis and underlying resistance mechanisms in clinical strains, Journal of Fungi (Basel). 10;6(1), 2020.

Salazar, S.B., Wang, C., Musterkotter, M., Okamoto, M., Takahashi-Nakagchi, A., Chibana, H., Lopes, M., Güldener, U., Butler, G. and Mira, N., “Comparative genomic and transcriptomic analyses unveil novel features of azole resistance and adaptation to the human host in Candida glabrata”, FEMS Yeast Research 1;18(1), 2018.

 

Oral Communications

Salazar, S.B., Bernardo, R., Cunha, D.V., Wang, C., Chibana, H., Silva, S., Pereira, L., Sá-Correia, I., Azeredo, J., Butler, G., Mira, N.P., “Genetic adaptive mechanisms mediating response ans tolerance to acetic acid stress in the human pathogen Candida glabrata: role of the CgHaa1-dependent signalling pathway”, Microbal Stress Meeting: Systems to Molecules and Back, 23-25 April 2018, Kinsale, Ireland(Best talk by an Early Career Researcher sponsored by the journal Genes)

Salazar, S.B., Pedreira, T., Chibana, H., Lopes, M.M., Güldene, U., Mira, N.P., Genomic Adaptative Mechanisms mediating Azole Resistance and Adaptation to the Human Host in Candida glabrata, with emphasis on the role of CgPDR1 regulon”, XXI Jornadas de Biologia de Leveduras, 8-9 June 2018, Braga, Portugal

 

Poster Communications

Salazar, S.B., Pedreira, T., Henriques, R., Wang, C., Musterkotter, M., Okamoto, M., Takahashi-Nakaguchi, A., Chibana, H., Lopes, M.M., Güldener, U., Butler, G., Mira, N.P., “Comparative genomic and transcriptomic analysis unveil novel features of azole resistance and adaptation to the human host in Candida glabrata”, MicroBiotec2017 December 7-8, 2017, Porto, Portugal

Salazar, S., Silva, S., Mil-Homens, D., Pimenta, A.I., Chibana, H., Azeredo, J., Fialho, A.M., Mira, N.P., The Candida glabrata CgHaa1-dependent system is required for biofilm formation, adhesion to epithelial cells and maximal virulence against Galleria mellonela”, Advanced Lecture Course on Human Fungal Pathogens, 18-24 May 2019, Nice, France

 

DOCTORAL PROGRAM (36 ECTS)

• General Doctoral Training (6 ECTS)

• Advanced Experimental Techniques and methodologies (6 ECTS)

• Bioentrepreneurship (6 ECTS)

• Outreach and Teaching Skills (6 ECTS)

• Advanced Topics in bioengineering and Biological Sciences (6 ECTS)

• Gene therapy (6 ECTS)