Jorge João

Class 2019
PhD Grant:  PD/BD/150335/2019

 

PhD thesis

Cell-Free Biomanufacturing of Protein Nanocages for Drug Delivery

 

Supervisor

• Miguel F. Prazeres, Professor @ IST/ULisboa, PT

 

Proposal summary

Protein nanocages are versatile vehicles for drug delivery. These nanometer scale architectures feature an inner cavity that can be loaded with different drugs. The clinical development of protein nanocages requires large amounts of pure, well-folded assemblies. While cell-based biomanufacturing works well at lab scale, cell-free synthesis is an attractive option for the rapid production of small batches. The major goal of this project is to develop scalable and cost-effective cell-free processes for the biomanufacturing of protein nanocages. A heat shock protein and ferritin will be used as model nanocages. Production of nanocages in E. coli cells and subsequent purification will be set-up and used as benchmark. Cell-free synthesis of the nanocages will be developed and compared with in vivo production. Characterization of nanocages will include in vitro internalization cell assays to check biological activity.

 

PUBLICATIONS

Papers

João J, Lampreia J, Prazeres DMF, Azevedo AM, Manufacturing of bacteriophages for therapeutic applications, Biotechnology Advances, 49:107758, 2021, doi:10.1016/j.biotechadv.2021.107758

 

Oral Presentations

João J, Rosa SS, Azevedo AM, Prazeres DMF, Chromatographic purification of small heat shock protein nanocages, 40th ISPPP – International Symposium on the Separation of Proteins, Peptides and Nucleotides, November 7-10, 2021, Porto, Portugal

 

Poster Presentations

João J, Rosa SS, Azevedo AM, Prazeres DMF, Development of a chromatographic approach for the purification of small heat shock protein nanocages, 3rd BioIberoAmerica – Ibero-American Congress on Biotechnology, April 7-9, 2022, Braga, Portugal

João J, Rosa SS, Azevedo AM, Prazeres DMF, A chromatographic strategy for the purification of small heat shock protein nanocages, MICROBIOTEC’21  Congress of Microbiology and Biotechnology 2021, November 23-26, 2021, virtual mode

João J, Rosa SS, Azevedo AM, Prazeres DMF, Purification of small heat shock protein nanocages through a chromatographic approach, PhD Open Days 2021, November 22-24, 2021, Instituto Superior Técnico, Lisbon, Portugal

João J, Rosa SS, Azevedo AM, Prazeres DMF, Chromatographic purification of small heat shock protein nanocages, 15th SBCN – International Symposium on Biochromatography and Nanoseparations, May 17-20, 2021, virtual mode (recognized with the Poster Prize – VIJI Awards SBCN 2021)

 

DOCTORAL PROGRAM (36 ECTS)

Curricular Units:

• General Doctoral Training (6 ECTS)

• Advanced Experimental Techniques and methodologies (6 ECTS)

• Bioentrepreneurship (6 ECTS)

• Molecular and Cell Biophysics (6 ECTS)

• Nanobiotechnology (6 ECTS)

• Outreach and Teaching Skills – Department of Bioengineering (6 ECTS)

Advanced courses:

• Course “Scientific Tools in Biological Assays: Principles of Cell Cultures and Genetics”, organized in virtual mode by the Faculty of Pharmacy – University of Lisbon, March 17-19, 2022 (22 hours)

• TIMB3 Workshop “Imaging in Biosciences”, organized at ITQB NOVA, February 7, 2022, Oeiras, Portugal (9 hours)

• SPICA Virtual Session “The Importance of Chromatography in the Development of Vaccines”, organized in virtual mode, October 14, 2021 (2 hours)

• Workshop “Escrita Científica – Da observação da realidade à publicação de um artigo: um processo e um resultado”, organized in virtual mode by the Library of Instituto Superior Técnico, June 15-24, 2021 (6 hours)

• Técnico Career Workshop “How to build a CV by BCG”, organized in virtual mode by the Technology Transfer Office – Instituto Superior Técnico, April 20, 2021 (2 hours)

• Técnico Career Workshop “LinkedIn (Básico) by Deloitte”, organized in virtual mode by the Technology Transfer Office – Instituto Superior Técnico, April 5, 2021 (2 hours)

• Workshop “How to Prepare an Excellent Presentation (or at Least an Acceptable One)?”, organized in virtual mode within the PhD Open Days 2020 – Instituto Superior Técnico, October 28, 2020 (3 hours)

• Cell Culture-based Viral Vaccines Course, 5th edition, organized in virtual mode by the European Society of Animal Cell Technology (ESACT), September 28 – October 2, 2020 (25 hours)

• 17th International PhD Student Symposium “Horizons in Molecular Biology”, organized in virtual mode, September 14-17, 2020 (13 hours)

• Champalimaud Research Symposium 2019 – Tissue Environment in Health and Disease, sponsored by the International Union of Biochemistry and Molecular Biology (IUBMB), October 8-10, 2019, Lisbon, Portugal (18 hours)

• Workshop “Teaching STEM Students – Transformation Guide for Teaching Assistants, 3rd Edition”, organized by the Academic Development Office – Instituto Superior Técnico, September 18-19, 2019, Lisbon, Portugal (6 hours)