Ana Isabel Matos – AIM Fellow 2015

Ana Isabel Matos_AIM 2015

Ana Isabel Matos has a bachelor degree in Biochemistry from the Faculty of Sciences and Technology of the New University of Lisbon and a master degree in Biochemistry from the Faculty of Sciences of the University of Lisbon. Her PD-FCT AIM project is entitled “Combinatorial multivalent nanoplatform for colorectal cancer treatment”. This project takes place at the Faculty of Pharmacy of the University of Lisbon and INESC MN. The thesis is supervised by Helena Florindo (Faculty of Pharmacy of the University of Lisbon) and co-supervised by João Pedro Conde (INESC MN and Department of Bioengineering of Técnico). Her doctoral program started on October 1st 2015. Here is an abstract of Ana Isabel’s PhD project:

ABSTRACT

Per year, colorectal cancer (CRC) is responsible for 655,000 deaths throughout the world. Almost 70 % of CRC patients are diagnosed with metastatic disease, mainly hepatic metastases, and present a very low 5 -years survival rate. The heterogeneity of cancer cells has impaired the successful outcome of several therapeutic approaches, including surgery, chemotherapy and radiotherapy, requiring effective combinatorial approaches. Immunotherapeutic approaches have shown very positive outcomes in other disseminated malignant diseases. However, an effective strategy against the heterogeneous population of cancer cells requires a combinatory approach to modulate different cells and mechanisms involved in tumor growth and dissemination. COMBI-CRC will tackle this goal by developing a combinatorial multivalent nanoplatform for CRC immunotherapy and drug delivery. COMBI-CRC will comprise two types of polymeric nanoparticles able to carry different active molecules and conjugate engineered targeting moieties to in vivo target and modulate distinct cell populations:

  • Nano_1: Immunotherapy based on dendritic cells (DCs)-targeted NPs,
  • Nano_2: Drug delivery based on tumor microenvironment (TME)-targeted NPs.

Additionally, the cross talk between immune cells and CRC cells (CRCC) will also be assessed by reconstituting complex immune and tumor microenvironments on microfluidic chips. A simple microfluidic device will also be exploited to investigate the crosstalk between metastatic CRCC and T lymphocytes from defective immune system, to reproduce T lymphocytes-mediated antitumor activity and CRCC-mediated tumor metastasis. The contact and the release of soluble mediators between malignant and immune cells are crucial to mediate tumor growth and metastasis, in a process termed immunosurveillance. However, immunosurveillance mechanisms fail in conditions of immunodeficiency. Microfluidic chips have been developed to follow unexplored cell-cell interactions by co-culturing several types of cells, transposing in vivo studies to a second generation of in vitro smart environments. This highly innovative and technologically advanced chemically-defined nanoplatform will lead to a safe multivalent nanomedicine able to combine the effect of a cytotoxic drug at cancer site with a balanced and multi-targeted immunotherapy, which overall outcome can constitute a real hope for patients with metastatic CRC disease.

ADDITIONAL INFO (December, 2017)

FELLOWSHIPS, GRANTS AND AWARDS (in bold, prior to AIM)

2017     Travel awardfrom TTP Labtech to attend the GRC Cancer Nanotechnology – Multimodal Nano-Therapies Against Cancer in Mount Snow, West Dover (VT), USA (18 – 23 June 2017).

2017     Free registration award from EACR – European Association for Cancer Research to attend the Accelerating Cancer Immunotherapy Conference in London, United Kingdom (29 – 30 March 2017).

2015     PhD research grant (PD/BD/113959/2015) from Fundação para a Ciência e a Tecnologia (FCT), Portugal / AIM – Avanced integrated Microsystems Doctoral Program (IST).

2015     Research project and Fellowship (PGG/037/2014) from Programa Gilead Génese, Portugal.

2014     Fellowship (PTDC/SAU-FAR/119389/2010) from Fundação para a Ciência e a Tecnologia (FCT), Portugal.

2013     Fellowship (PTDC/AGR-AAM/102821/2008) from Fundação para a Ciência e a Tecnologia (FCT), Portugal.

2012     IAESTE traineeship from IAESTE Portugal.

2011     Fellowship (PTDC/QUI/66612/2006) from Fundação para a Ciência e a Tecnologia (FCT), Portugal.

PARTICIPATION IN SCIENTIFIC PROJECTS

  • Euronanomed II Program – 2015 – Joint Translational Call for Proposals for European Innovative Research & Tecnhological Development Projects in Nanomedicine. FCT funded research project entitled “NanoTumin – Targeting tumor microenvironment by a translational multivalent nanomedicine: towards an effective anticancer combination immunotherapy” (ENMed/0003/2015), Team member, 2016 – 2019.
  • UT Austin Portugal Program – 2014 – Call for Scientific Research and Technology Development Projects. FCT funded research project entitled “Multidisciplinary strategy to develop novel multicomponent nanoscale systems for immune modulation” (UTAP-ICDT/DTP-FTO/0016/2014), Team member, 2015 – 2018.

PUBLICATIONS (in bold, prior to AIM)

  • Ana L. Silva, C. Peres, J. Conniot, Ana I. Matos, L. Moura, B. Carreira, V. Sainz, A. Scomparin, R. Satchi-Fainaro, V. Préat, H. F. Florindo. Nanoparticle impact on innate immune cell pattern-recognition receptors and inflammasomes activation. Seminars in Immunology, 2017, 34:3-24 (DOI: 10.1016/j.smim.2017.09.003).
  • Ana L. Silva, L. Moura, B. Carreira, J. Conniot, Ana I. Matos, C. Peres, V. Sainz, L. C. Silva, R. S. Gaspar, H. F. Florindo. Functional moieties for intracellular traffic of nanomaterials. In: Biomedical Applications of Functionalized Nanomaterials, 2017, Chapter 14 (Sarmento and das Neves, Ed., Elsevier).
  • Santos F, Matos AI, Ventura AE, Gonçalves J, Veiros LF, Florindo HF and Gois PMP. Modular Assembly of Reversible Multivalent Targeting Drug Conjugates. Angew Chem Int Ed Engl, 2017 (DOI: 10.1002/anie.201703492).
  • Peres C, Matos AI, Viana AS, Graça L, Préat V, Florindo HF. ML-07.01.3-001: Nanoparticle-based cancer vaccine to target dendritic cells and the tumor microenvironment. The FEBS Journal, 2016, 283 (Suppl.1): 12-54 (DOI: 10.1111/febs.13805).
  • Peres C, Matos AI, Conniot J, Zupancic E, Sainz V, Silva J, Florindo HF. Poly(lactic acid)-based nanosystems are promising tools for immune modulation. Acta Biomaterialia, 2016, 48:41-57 (DOI: 10.1016/j.actbio.2016.11.012).
  • Zupančič E, Peres C, Matos AI, Lopes J, Moreira JN, Gaspar RS, Florindo HF. “Translational Peptide-associated Nanosystems: Promising Role as Cancer Vaccines”. Current Topics in Medicinal Chemistry, 2016, 16(3):291-313. (DOI: 10.2174/1568026615666150701115508).
  • Santos F, Rosa J, Candeias N, Carvalho C, Matos AI., Ventura AE., Florindo HF, Silva LC, Pischel U and Gois PMP, “A Three-Component Assembly Promoted by Boronic Acids Delivers a Modular Fluorophore Platform (BASHY Dyes)”, Chemistry – A European Journal, 2016, 22(5): 1631-7 (DOI: 10.1002/chem.201503943).
  • Sainz V, Conniot J, Matos AI, Peres C, Moura L, Florindo HF, Gaspar RS. “Regulatory aspects on nanomedicines”. Biochemical and Biophysical Research Communications, 2015, 468(3): 504-5010 (DOI: 10.1016/j.bbrc.2015.08.023).
  • A. S. N. Formagio, M. C. Vieira, C. R. F. Volobuff, M. S. Silva, A. I. Matos, C. A. L. Cardoso, M. A. Foglio, J. E. de Carvalho, “In vitro biological screening of the anticholinesterase and antiproliferative activities of medicinal plants belonging to Annonaceae”, Brazilian Journal of Medical and Biological Research 48 (2015) 308-315 (DOI: 10.1590/1414-431X20144127; Q1 on Pharmacology, Toxicology and Pharmaceutics and Q2 on Medicine).
  • A. S. N. Formagio, T. E. Masetto, M. C. Vieira, N. A. H. Zárate, A. I. N. de Matos, C. R. F. Volobuff, “Allelopathic and antioxidant potential of plants extracts”, Bioscience Journal 30 (2014) 629-638 (ISSN: 1981-3163 – Online Journal; ISSN: 1516-3725 – Printed Journal; Q2 on Agricultural and Biological Sciences).
  • Yu Niu, Ana I. Matos, Luisa M. Abrantes, Ana S. Viana, Gang Jin, “Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing”, Langmuir  28 (2012) 17718-17725 (DOI: 10.1021/la303032f; Q1 on Medicine, Electrochemistry and Surfaces and Interfaces).

ORAL COMMUNICATIONS (in bold, prior to AIM)

  • Sainz V, Peres C, Moura L, Matos AI, Viana AS, Lopes JA, Gaspar MM, Brocchini S, Zloh M, Florindo HF. A novel nanoplatform as metastatic melanoma cancer vaccine. 1st SPLC-CRS Young Scientist Meeting, Santiago de Compostela, Spain, April 2017.
  • Peres C, Matos AI, Viana AS, Graça L, Préat V, Florindo HF. Nanoparticle-based cancer vaccine to target dendritic cells and the tumor microenvironment. 41st Federation of European Biochemical Societies (FEBS) congress, Ephesus/Kuşadası, Turkey, September 2016 (cancelled due to external factors).
  • Peres C, Matos AI, Viana AS, Graça L, Préat V, Florindo HF. Nanoparticle-based cancer vaccine to target dendritic cells and the tumor microenvironment. 16th FEBS Young Scientists’ Forum, Ephesus/Kuşadası, Turkey, September 2016 (cancelled due to external factors).
  • Matos AI, Peres C, Ventura AE, Viana AS, Borrego P, Silva LC, Taveira N, Florindo HF. Development of polymeric vaccine delivery nanosystems for HIV/AIDS control through dendritic cell modulation. XI Spanish-Portuguese Conference on Controlled Drug Delivery – Revolutionary Approaches in Nanomedicine Development, Granada, Spain, January 2016.
  • Peres C, Matos AI, Viana AS, Graça L, Préat V, Florindo HF. Polymeric nanoparticle-based vaccine: targeting dendritic cells and the tumor microenvironment. XI Spanish-Portuguese Conference on Controlled Drug Delivery – Revolutionary Approaches in Nanomedicine Development, Granada, Spain, January 2016.
  • Peres C, Matos AI, Conniot J, Sainz V, Graça L, Préat V, Florindo HF. Multicomponent nanoparticles for vaccine delivery and immunomodulation. Molecular and Supramolecular Carriers for Imaging and Therapy Conference, FP7-PEOPLE-2012-ITN - Marie-Curie Action: “Initial Training Networks”, Lisbon, Portugal, July 2015.
  • A. I. MATOS, A. S. N. FORMAGIO, M. C. VIEIRA. Phenols and flavonoids content and antioxidant activity of seven plant extracts. 3rd Encontro de Ensino de Graduação, 5th Encontro de Pós-Graduação, 6th Encontro de Iniciação Científica and 6th Encontro de Extensão da UFGD, Dourados, Mato Grosso do Sul, Brazil, October 2012.
  • A. I. Matos, I. Almeida, Y. Niu, G. Jin, L. M. Abrantes, A. S. Viana. New gold biosensor interfaces with ability to reduce non-specific adsorption. 63rd Annual Meeting of the International Society of Electrochemistry, Prague, Czech Republic, August 2012.
  • Yu Niu, Ana Viana, A. I. Matos, Luisa Maria Abrantes, Gang Jin. Antibody immobilization on carbon disulfide modified gold surface and Its biosensing application. World Congress 2012, Beijing, China, May 2012.

POSTER COMMUNICATIONS (in bold, prior to AIM)

  • Matos AI, Peres C, Moreiras H, Viana AS, Graça L, Barral D, Conde JP, Florindo HF. Multivalent polymeric nanoparticles for colorectal cancer immunotherapy and immunomodulation. 9th iMed.ULisboa Postgraduate Students Meeting & 2nd i3DU Meeting, Lisbon, Portugal; July 2017.
  • Peres C, Moura L, Matos AI, Sainz V, Viana AS, Gaspar RS, Graça L, Préat V, Florindo H. Polymeric nanoparticles as cancer vaccines and immunomodulators. 9th iMed.ULisboa Postgraduate Students Meeting & 2nd i3DU Meeting, Lisbon, Portugal; July 2017.
  • Matos AI, Peres C, Moreiras H, Viana AS, Graça L, Barral D, Gaspar R, Conde JP, Florindo HF. Multivalent polymeric based biodegradable nanoparticles for colorectal cancer immunotherapy and immunomodulation. GRC Cancer Nanotechnology – Multimodal Nano-Therapies Against Cancer; Mount Snow, West Dover (VT), USA; June 2017
  • Peres C, Moura L, Matos AI, Sainz V, Viana AS, Gaspar RS, Graça L, Préat V, Florindo H. Polymeric nanoparticles as breast cancer vaccines and immunomodulators. GRC Cancer Nanotechnology – Multimodal Nano-Therapies Against Cancer; Mount Snow, West Dover (VT), USA; June 2017.
  • Moura L, Duro-Caetano A, Peres C, Gallon E, Sainz V, Matos A, Gaspar G, Vicente MJ, Florindo HF. A novel multifunctional star-shaped polypeptide-based platform as an anti-cancer immunotherapeutic approach. GRC Cancer Nanotechnology – Multimodal Nano-Therapies Against Cancer; Mount Snow, West Dover (VT), USA; June 2017.
  • Helena F Florindo, Eva Zupančič, Carina Peres, Vanessa Sainz, Ana I Matos, Liane Moura, Caterina Curato, Eilam Yeini, Steffen Jung, and Ronit Satchi-Fainaro. Multicomponent Nanoscale Systems for Immune Modulation Against Solid Tumors. Frontiers in Cancer Immunotherapy, New York, USA, February 2017.
  • Carina Peres, Vanessa Sainz, Ana I. Matos, Ana S. Viana, Liane Moura, Luís Graça, Luís Graça, Véronique Préat and Helena F Florindo. Multicomponent aliphatic polyester-based nanoparticles for antigen delivery and immune modulation against cancer disease.NanoBio&Med2016 Conference, Barcelona, Spain, November 2016.
  • PT Team: Carina Peres, Ana I. Matos, Ana C. Santos, Ana S. Viana, João Gonçalves, Luís Graça, Rogério Gaspar, Véronique Préat, Helena Florindo. UT Austin Team: Angela M. Wagner, Julia Vela, Nicholas A. Peppas. Polymeric nanoparticle-based vaccine to target dendritic cells and the tumor microenvironment. UT Austin | Portugal Annual Conference, Lisbon, Portugal, May 2016.
  • Peres C, Matos AI, Viana AS, Graça L, Préat V, Florindo HF. Polymeric nanoparticle-based vaccine to target dendritic cells and the tumor microenvironment. 14th CIMT Annual Meeting, Mechanisms of efficacy in cancer immunotherapy, Mainz, Germany, May 2016.
  • V. Sainz, A. I. Matos, Ana S. Viana, J. A. Lopes, S. Brocchini, M. Zloh, H. F. Florindo. Promising melanoma therapeutic cancer vaccine based on hybrid lipid-polymeric nanoparticles. 14thCIMT Annual Meeting, Mechanisms of efficacy in cancer immunotherapy, Mainz, Germany, May 2016.
  • Peres C, Matos AI, Viana AS, Graça L, Préat V, Florindo HF. Polymeric nanoparticle-based vaccine to deliver tumor-associated antigens and for immunomodulation. 2nd ASPIC International Congress, Porto Cancer Meeting Conference, Porto, Portugal, April 2016.
  • V. Sainz, A. I. Matos, Ana S. Viana, J. A. Lopes, S. Brocchini, M. Zloh, H. F. Florindo. Hybrid lipid-polymeric nanoparticles as cancer immunotherapeutics.2nd ASPIC International Congress, Porto Cancer Meeting Conference, Porto, Portugal, April 2016.
  • A. I. Matos, C. Peres, A. E. Ventura, Ana S. Viana, P. Borrego, P. Góis, L. C. Silva, N. Taveira and H. F. Florindo. Development of PLGA-based nanocariers for Human Immunodeficiency Virus Type 1 vaccine delivery. Molecular and Supramolecular Carriers for Imaging and Therapy Conference, FP7-PEOPLE-2012-ITN - Marie-Curie Action: “Initial Training Networks” Lisbon, Portugal, July 2015.
  • Ana I. Matos, Ana S. Viana, Carla Real, Nuno Taveira, Helena F. Florindo. Development of polymeric nanocarriers for Human Imunodeficiency Virus type 1 vaccine delivery. 6th iMed.ULisboa Postgraduate Students Meeting, Lisbon, Portugal, July 2014.
  • Ana I. Matos, M. M. Rocha, M. M. M. Neto, L. L. Martins, M. P. Mourato. Determination of plant-toxicity inductors metals at a rotating-disc bismuth film electrode by anodic stripping voltammetry. III Jornadas de Electroquímica e Inovação: Nanomateriais e Electroquímica, Vila Real, Portugal, September 2013.
  • Ana Isabel Neves de Matos, Anelise Samara Nazari Formagio, Maria do Carmo Vieira, Carla Roberta Ferreira Volobuff. Phenols and Flavonoids content in methanolic extract of Barbatimão (Stryphnodendron adstringens). VII Encontro de Iniciação Científica, IV Salão de Pesquisa Docente e II Mostra de Pós-Graduação – Unigran, Dourados, Mato Grosso do Sul, Brazil, September 2012.
  • Inês Almeida, Ana I. Matos, Ana S. Viana. Modified electrodes with functionalized nanoparticles assembled using in-situ dithiocarbamate chemistry. 3rd International Symposium on Surface Imaging/Spectroscopy at the Solid/Liquid Interface, Cracow, Poland, May 2012.

SUBJECTS

  • Bioentrepreneurship
  • Advanced topics on Bioengeneering and Biological Sciences replaced by i3DU Course Advanced Drug Delivery, 14-18 March 2016.
  • Teaching and Scientific Disclosure: support on Cell and Tissue Engeneering practical classes

COURSES AND WORKSHOPS

  • Statistics for Begginers Course certified by Instituto de Medicina Molecular (IMM), Lisboa, Portugal, October – December 2015
  • Laboratory Animal Science Course (according to FELASA cat. B) certified by Instituto de Medicina Molecular (IMM), Lisboa, Portugal, April – May 2016.
  • 1st FLxFlow Course – Principles and Applications of Flow Cytometry (1st FLxFlow Course – Principles and Applications of Flow Cytometry certified by Instituto de Medicina Molecular (IMM), Lisboa, Portugal, 16 – 20 October 2017.
  • Microfabrication Workshop – Microfluidics Fabrication for Biorecognition Detection at INESC-MN, IST, Lisboa, Portugal, 15 – 17 November 2017.