Pedro Fontes – AIM Fellow 2017


Background & Project

Pedro Mendes Fontes has a bachelor degree in Biochemistry from Faculdade de Ciências e Tecnologia of NOVA University of Lisbon and a master degree in Biotechnology from Instituto Superior Técnico of University of Lisbon. Also has a Laboratory Animal Science Course (according to FELASA cat. B) from Instituto de Medicina Molecular of University of Lisbon.

His PD-FCT AIM project is entitled “Discovery-On-Chip: integrated microfluidic system for antibody discovery” and will take place at Faculdade de Farmácia of the University of Lisbon place and INESC MN.

The thesis is supervised by João Gonçalves (Faculdade de Farmácia of University of Lisbon) and co-supervised by João Pedro Conde (INESC MN and Department of Bioengineering of Instituto Superior Técnico) and by. His doctoral program started on October 1st, 2017.

Project Goals

Development of a Phage Display Chip

Development of a microfluidic platform for the discovery, selection and isolation of antibodies against relevant colon cancer stem cell (CCSCs) biomarkers;

Discovery of Relevant Colon Cancer Stem Cell Biomarkers as Novel Targets for Cancer Therapy

The discovery of relevant biomarkers against CCSCs, particularly biomarkers that would allow the internalization of antibodies. These would allow the creation of new strategies to tackle CCSCs, thus improving colon cancer diagnosis and therapy;

Discovery of Internalizing Antibodies for the Delivery of Therapeutics to Colon Cancer Stem Cells

The discovery of internalizing antibodies is of the utmost importance to the development of antibody-drug conjugates (ADCs), an innovative therapeutic approach for cancer.

Ongoing Activities

- Optimization of mammalian cell culture on-chip;

- Phage detection on-chip using fluorescence microscopy;

- Cell-phage dissociation on-chip.

Past Activities

- Completed the courses of Nanobiotechnology and Bioentrepreneurship, Instituto Superior Técnico (2018);

- Attended a course on BioPharmaceuticals and Advanced Therapies, Faculdade de Farmácia (2018).

Publications (prior to AIM)

Joana R Sousa, MSc; Célia M Silveira, PhD; Pedro Fontes, MSc; Catarina Roma-Rodrigues, PhD; Alexandra R Fernandes, PhD; Gonzalez Van Driessche; Bart Devreese, PhD; Isabel Moura, PhD; José J Moura, PhD; M. Gabriela Almeida, Ph.D, Understanding the response of Desulfovibrio desulfuricans ATCC 27774 to different electron acceptors – biosynthetic costs modulate substrate selection