Ricardo Fradique – AIM Fellow 2015

Ricardo Fradique_AIM 2015

Ricardo Fradique  has undergraduate degrees (bachelor and master) in Biomedical Sciences from the Universidade da Beira Interior (Covilhã, Portugal).

His PD-FCT AIM project is entitled “Design of a miniaturized platform for biopharmaceuticals production”. This project takes place at IBB and INESC MN. The thesis is supervised by Raquel Barros (IBB and Department of Bioengineering of Técnico) and co-supervised by João Pedro Conde (INESC MN and Department of Bioengineering of Técnico). His doctoral program started on November 1st 2015.

Project goals

The main goal of this doctoral project is to miniaturize and integrate the different stages of a biomanufacturing process, in particular the upstream cell culture and downstream product recovery and purification. This is divided into different milestones:

- Development of a microfluidic lysis screening chip

- Integration of a microfluidic lysis method with an aqueous two phase system for product concentration and purification

- Development of a microfluidic chemostat

- Integration of the multiple on-chip methods on a single chip

- Characterization and process modelling of the final microfluidic chip for information extraction regarding larger scale applications

 Ongoing activities

- Integration of an aqueous two phase system with a lysis method on-chip

- Development of a microfluidic chemostat

Oral comunications

- Poster Presentation at the Microbiotec conference entitled: “Continuous cell concentration and lysis using inertial microfluidics”; 7/12-9/12/2017

- Oral Presentation at the Anything But Conventional Chromatography Workshop Organized by ESBES, entilted: “Inertial microfluidics for continuous cell concentration and lysis quantification”, 24/11/2017

- Oral Presentation at the European Symposium on Biochemical Engineering Sciences, entitled “Microfluidic platform for bacterial cell lysis and protein concentration”, 9/9-12/9/2018

Teaching and divulgation activities

- Supervision of an International Bachelors Student: Niklas Floto (2018)

- Supervision of a Master Student: Andreia Jardim (2018)


- 1st Eurombr International Training Course, Braunschweig, Germany, on Applications of microbioreactors in bioprocess development

- Summer School at the University of Applied Sciences Kaiserslautern, Germany, on pressure sensor microfabrication

- Completed the courses of Animal Cell Technology, Bioentrepreneurship, Basic Doctoral Formation, Outreach and Teaching Skills, and Nanobiotechnology(IST).