Sílvia Andreia Bento da Silva Sousa

(Post-Doc Researcher) *

Alternative Pages: Google Scholar; Research ID; iBB webpage

Status at iBB - Institute for Bioengineering and Biosciences:

Education:

  • PhD in Biotechnology, IST, UTL, Lisbon, Portugal, 2007 (supervision: J.H. Leitão)

  • Graduation in Biology,University of Lisbon, Portugal, 2001

  • Research Training during the last year of graduation in Biology (2000/2001) at Grupo de Ciências Biológicas do CEBQ, Instituto Superior Técnico

Positions:

  • Post-Doc Researcher, CEBQ/BSRG, IST, 2007 - present

Research Activities:

  • Physiology and Molecular Biology of Bacterial Exopolysaccharide Biosynthesis

  • Identification and characterization of virulence factors of bacteria of the Burkholderia cepacia complex (Bcc)

  • Role of small RNAs on the virulence of Bcc

  • Molecular characterization of microbial populations from sludge bed reactors for wastewater treatment

Grants:

Publications:

Thesis

Sílvia Sousa, "Burkholderia cepacia complex infections in cystic fibrosis patients: biosynthesis and role of the exopolysaccharide and investigation of other potential virulence factors", Tese de Doutoramento (Ph.D. thesis), Instituto Superior Técnico, 2007

Sílvia S. Sousa, "Papel do exopolissacárido nas infecções respiratórias por Burkholderia cepacia em doentes com Fibrose Quística", Research Training Thesis (last year of graduation in Biology, University of Lisbon), Lisbon, 2001.

Papers in international journals with referees:

Sousa, S.A., Feliciano, J.R., Pita, T., Guerreiro, S.I., Leitão, J.H., "Burkholderia cepacia complex virulence gene expression regulation: a review", Genes 8:43, 2017. DOI: 10.3390/genes8010043

Moreira, A.S., Mil-Homens, D.*, Sousa, S.A.*, Coutinho, C.P.*, Pinto-de-Oliveira, A., Ramos, C.G., dos Santos, S.C., Fialho, A.M., Leitão, J.H., Sá-Correia, I., Variation of Burkholderia cenocepacia virulence potential during cystic fibrosis chronic lung infection. Virulence, doi:10.1080/21505594.2016.1237334 (in press).

Sousa SA, Morad M, Feliciano JR, Pita T, Nady S, El-Hennamy RE, Abdel-Rahman M, Cavaco J, Pereira L, Barreto C, Leitão JH., "The Burkholderia cenocepacia OmpA-like protein BCAL2958: identification, characterization, and detection of anti-BCAL2958 antibodies in serum from B. cepacia complex-infected Cystic Fibrosis patients." AMB Express. 6(1):41, 2016.

Sousa SA, Leitão JH, Martins RC, Sanches JM, Suri JS, Giorgetti A., "Bioinformatics Applications in Life Sciences and Technologies", Biomed Res Int., doi: 10.1155/2016/3603827.

Feliciano, J.R., Grilo, A.M., Guerreiro, S.I., Sousa, S.A., Leitão, J.H., "Hfq: a multifaceted RNA chaperone involved in virulence", Future Microbiology, 11(1):137-51, 2016.

Ramos, C.G., Grilo, A.M., Sousa, S.A., Feliciano, J.R., da Costa, P.J., Leitão, J.H., "Regulation of Hfq mRNA and protein levels in Escherichia coli and Pseudomonas aeruginosa by the Burkholderia cebocepacia MtvR sRNA", PLoS One, 9(6): e98813, 2014.

Sousa, S.A., Feliciano, J.R., Grilo, A.M., Leitão, J.H., "Bioinformatics: a molecular microbiologist's perspective", Current Bioinformatics, 9: 8-17, 2014.

Sousa, S.A., Feliciano, J.R., Pinheiro, P.F., Leitão J.H., "Biochemical and functional studies on the Burkholderia cepacia complex bceN gene, encoding a GDP-mannose 4,6-dehydratase", PLoS One. 2013;8(2):e56902, 2013.

H. Nadais, M. Barbosa, I. Capela, L. Arroja, C.G. Ramos, A. Grilo, S.A. Sousa, J.H. Leitão, Enhancing Wastewater Degradation and Biogas Production by Intermittent Operation of UASB Reactors. Energy. 36: 2146-68, 2011.

S.A. Sousa, C.G. Ramos, J.H. Leitão, Burkholderia cepacia Complex: emerging multihost pathogens equipped with a wide range of virulence factors and determinants. International Journal of Microbiology. ID 607575, 2011.

C.G. Ramos, S.A Sousa, A.M. Grilo, L. Eberl, J.H. Leitão, The Burkholderia cenocepacia K56-2 pleiotropic regulator Pbr, is required for stress resistance and virulence. Microbial Pathogenesis. 48: 168-177, 2010.

J.H. Leitão, S A Sousa, A.S. Ferreira, C.G. Ramos, I.N. Silva, L.M. Moreira. Pathogenicity, virulence factors, and strategies  to fight against Burkholderia cepacia complex pathogens and related  species. Applied Microbiology and Biotechnology. DOI:  10.1007/s00253-010-2528-0

S.A. Sousa, C.G. Ramos, L.M. Moreira, J.H. Leitão, The hfq gene is required for stress resistance and full virulence of Burkholderia  cepacia to the nematode Caenorhabditis elegans. Microbiology  156:896-908, 2010.

C.G. Ramos, A.M. Grilo, S.A. Sousa, M.L. Barbosa, H. Nadais, J.H. Leitão,  A new methodology combining PCR, cloning, and sequencing of clones  discriminated by RFLP for the study of microbial populations:  application to an UASB reactor sample. Applied Microbiology and  Biotechnology 85:801-806, 2010.

A.S. Ferreira, J.H. Leitão, I.N. Silva, P.F. Pinheiro, S.A. Sousa, C.G. Ramos, L.M. Moreira, Distribution of cepacian biosynthesis genes among environmental  and clinical Burkholderia strains and role of cepacian  exopolysaccharide in resistance to stress conditions. Applied and  Environmental Microbiology 76:441-450, 2010.

S.A. Sousa, C.G. Ramos, L. Almeida, L. Meirinhos-Soares, J. Wopperer, S. Schwager, L. Eberl, J.H. Leitão, " Burkholderia cenocepacia j2315 acyl carrier protein: a potential target for antimicrobials development?", Microbial Pathogenesis, 45, 331-336, 2008 (abstract)

S.A. Sousa, L.M. Moreira, J.H. Leitão, Functional analysis of the Burkholderia cenocepacia J2315 BceAJ protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities, Applied Microbiology and  Biotechnology, 80, 1015-1022, 2008 (abstract)

J.H. Leitão, S.A. Sousa, M.V. Cunha, M.J. Salgado, J. Melo-Cristino, M.C. Barreto and I. Sá-Correia, "Variation of the antimicrobial susceptibility profiles of Burkholderia cepacia complex clonal isolates obtained from chronically infected  cystic fibrosis patients: a five-year survey in the major Portuguese treatment center". European Journal of Clinical Microbiology & Infectious Diseases27, 1101-1111, 2008 (abstract).

S.A. Sousa, M. Ulrich, A. Bragonzi, M. Burke, D. Worlitzch, J.H. Leitão, C. Meisner, L. Eberl, I. Sá-Correia, G. Döring, "Virulence of Burkholderia cepacia complex strain in gp91phox-/- mice", Cellular Microbiology, 9, 2817-2825, 2007 (abstract)

S.A. Sousa, L.M. Moreira, J. Wopperer, L. Eberl, I. Sá-Correia, J.H. Leitão, "The Burkholderia cepacia bceA gene encodes a protein with phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activities", Biochemical and Biophysical Research Communications, 353, 200-206, 2007 (abstract)

A.S. Ferreira, J.H. Leitão, S.A. Sousa, A.M. Cosme, I. Sá-Correia, L.M. Moreira, "Functional Analysis of Burkholderia cepacia bceD and bceF Genes  Encoding a Phosphotyrosine Phosphatase and a Tyrosine Autokinase: Role in Exopolysaccharide Biosynthesis and Biofilm Formation", Applied and Environmental Microbiology, 73, 524-534, 2007 (abstract)

M.V. Cunha, S.A. Sousa, J.H. Leitão, L.M. Moreira, P.A. Videira, I. Sá-Correia, "Studies on the involvement of the exopolysaccharide produced by cystic fibrosis-associated isolates of the Burkholderia cepacia complex in biofilm formation and in persistence of respiratory infections", Journal of Clinical Microbiology, 42, 3052-3058, 2004 (abstract)

L.M. Moreira,  P.A. Videira, S.A. Sousa, J.H. Leitão, M.V. Cunha, I. Sá-Correia, "Identification and physical organization  of the gene cluster involved in the biosynthesis of Burkholderia cepacia complex exopolysaccharide", Biochemical and Biophysical Research Communications, 312, 323-333, 2003 (abstract

Abstracts in international journals with referees:

C.G. Ramos, S.A. Sousa and J.H. Leitão, "Identification and characterization of a phenazine biosythetic locus required for virulence of Burkholderia cenocepacia K56-2 to the nematode Caenorhabditis elegans", FEBS Journal, 275 (Suppl. 1) 275, 2008

 

A.S. Ferreira, J.H. Leitão, S.A. Sousa, A.M. Cosme, I. Sá-Correia, L.M. Moreira, J.H. Leitão, "Role of tyrosine kinase and a phosphatase in exopolysaccharide biosynthesis by Burkholderia cepacia", FEBS Journal, 274 (Suppl. 1), 223, 2007

 

M.V. Cunha, S.A. Sousa, J.H. Leitão, L.M. Moreira, P.A. Videira, I. Sá-Correia, "The exopolysaccharide from cystic fibrosis-associated Burkolderia cepacia complex bacteria: biosynthesis and possible involvement in the persistant of respiratory infections", Journal of Cystic Fibrosis, 4 (Suppl. 1), S45, 2005

D. Worlitzsch, S. Nonnenmacher, M. Ulrich, S. Sousa, J.H. Leitão, I. Sá-Correia, T. Coenye, P. Vandamme, G. Göring , "A rabbit antibody specific for exopolysaccharides of 10 different Burkholderia cepacia genomovars detects B. cepacia macrocolony formation in sputum of CF patients", Journal of Cystic Fibrosis, 2, S35, 2003

M.V. Cunha, J.H. Leitão, S. Sousa, L. Lito, M.J. Salgado, C. Barreto, I. Sá-Correia, "Persistent pulmonary infections with Burkholderia cepacia in patients from the major Portuguese cystic fibrosis center", Journal of Cystic Fibrosis, 1, S61, 2002

Oral Communications in Scientific Meetings

Ramos, C.G., Sousa, S.A., Grilo, A.M., da Costa, P.J.P., Feliciano, J.R, Leitão, J.H., A RNomics-based strategy identifies regulatory small RNAs in Burkholderia cenocepacia complex. First Portuguese Meeting in Bioengineering – IEEE-EMBS, 1-4 March 2011, IST, TagusPark, Oeiras, Portugal

 

Sousa, S.A., Ramos, C.G., Feliciano, J.R., Leitão, J.H., Identification and exploitation of Burkholderia cenocepacia complex virulence factors as potential antimicrobial targets. First Portuguese Meeting in Bioengineering – IEEE-EMBS, 1-4 March 2011, IST, TagusPark, Oeiras, Portugal

 

S.A. Sousa, C.G. Ramos, L. Soares, L. Eberl and J.H. Leitão, Burkholderia cenocepacia J2315 acp: Identification of a potential target for the development of new antimicrobials. XVI Congress of Biochemistry, Azores, 22-25 October 2008, Portugal

 

S.A. Sousa, C.G. Ramos, J.H. Leitão, "Burkholderia cenocepacia J2315 acyl carrier protein (ACP): a potential target for the development of antimicrobials against infections caused by Burkholderia species?", IUMS Congress, Istanbul, Turkey, 5-9 August 2008


Ana S. Ferreira, Jorge Leitão, Sílvia A. Sousa, Leonilde Moreira, "Extension of the bce cluster of genes involved in the biosynthesis of cepacian by the genus Burkholderia", International Burkholderia cepacia Working Group (IBCWG), Ca’ Tron di Roncade (Treviso), Italy, 14-17 April 2008

Silvia A. Sousa, Christian G. Ramos, Jorge H. Leitão, "The acyl carrier protein, involved in the virulence of Burkholderia cenocepacia J2315, is conserved within the Burkholderia genus", International Burkholderia cepacia Working Group (IBCWG), Ca’ Tron di Roncade (Treviso), Italy, 14-17 April 2008

SA Sousa, AS Ferreira, LM Moreira, G Doring, I Sá-Correia, JH Leitão, "Biosynthesis of the exopolysaccharide Cepacian, a virulence factor produced by bacteria of the Burkholderia cepacia complex", 7th International Meeting of the Portuguese Carbohydrate Group Glupor7, Oeiras, Portugal, 12-15th September, 2007

M.V. Cunha, A. Pinto-de-Oliveira, J.H. Leitão, S.A. Sousa, L. Meirinhos-Soares, C. Barreto, S. Correia, J. Melo-Cristino, M.J. Salgado, I. Sá-Correia, " An 11-year epidemiological and antimicrobial resistance survey of Burkholderia cepacia complex isolated in the major portuguese Cystic Fibrosis center", 11th Annual Meeting of the International Burkholderia cepacia Working Group, U. Michigan, Ann Arbor, USA, April, 2007

A.S. Ferreira, J.H. Leitão, S.A. Sousa, A.T. Granja, A. Popescu, A.M. Fialho, I. Sá-Correia, L.M. Moreira, "Exopolysaccharide biosynthesis and regulation in Burkholderia cepacia complex isolates", 11th Annual Meeting of the International Burkholderia cepacia Working Group, U. Michigan, Ann Arbor, USA, April, 2007

S.A. Sousa, A. Bragonzi, M. Ulrich, D. Worlitzsch, J.H. Leitão, M.C. Dinauer, E. Fiscarelli, L. Eberl, I. Sá-Correia, G. Doring, "The CGD mouse model for assessing Burkholderia cepacia complex virulence", 28th European Cystic Fibrosis Conference, Hersonissos, Crete, Greece, 22-25 June, 2005

S.A. Sousa, A. Bragonzi, M. Ulrich, D. Worlitzsch, J.H. Leitão, M.C. Dinauer, E. Fiscarelli, L. Eberl, I. Sá-Correia, G. Doring, "The CGD mouse model for assessing Burkholderia cepacia complex virulence", European Cystic Fibrosis Conference - New Frontiers in Basic Science of Cystic Fibrosis, Évora, Portugal, April 14-17, 2005

L.M. Moreira, J.H. Leitão, P.A. Videira, S.A. Sousa, I. Sá-Correia, "Physical organization and putative functions of the cluster of genes directing Burkholderia cepacia complex exopolysaccharide biosynthesis", XXXI Jornadas Portuguesas de Genética, Oeiras, Portugal, February 5-6, 2004

JH Leitão, MV Cunha, LM Moreira, PA Videira, SA Sousa, I Sá-Correia, "Burkholderia cepacia complex respiratory infections in cystic fibrosis patients: molecular epidemiology and biosynthesis and role of the exopolysaccharide", MICRO´03, Tomar,Portugal, 29/11 – 2/12, 2003


D Worlitzsch, S Nonnenmacher, M Ulrich, SA Sousa, JH Leitão, I Sá-Correia, T. Coenye, P. Vandamme, G. Döring, "A rabbit antiboby specific for exopolysaccharides of 10 different Burkholderia cepacia genomovars detects B. cepacia macrocolony formation in sputum of CF patients. 26th Congress European Cystic Fibrosis Society, Belfast, Ireland, June 2003

M.V. Cunha, J.H. Leitão, S.A. Sousa, L. Lito, M.J. Salgado, C. Barreto, I. Sá-Correia, "Persistent pulmonary infections with Burkholderia cepacia in patients from the major portuguese cystic fibrosis center", 25th European Cystic Fibrosis Conference, Genoa, Italy, 20-23 June, 2002

 

Poster presentations in Scientific Meetings

Ramos, C.G., Sousa,S.A., Feliciano, J.R., Grilo, A.M., Leitão, J.H., Functional analysis of the Burkholderia cenocepacia J2315 hfq2 gene encoding an unsual RNA chaperone, and identification of the small non-coding RNA LhdR, The Non Coding Genome EMBO-EMBL Symposium 2010, 13-16 October , Heidelberg, Germany

A.S. Ferreira, J.H. Leitão, I.N. Silva, S.A. Sousa, C.G. Ramos and L.M. Moreira. Extension of the bce cluster of genes involved in the biosynthesis of cepacian by the genus  Burkholderia. XVI Congress of Biochemistry, Azores, 22-25 October 2008, Portugal

S.A. Sousa, J.H. Leitão, "Cloning and characterization of Burkholderia cenocepacia J2315 outer membrane protein A (OmpA)-like protein", IUMS Congress, Istanbul, Turkey, 5-9 August 2008

S.A. Sousa, J.H. Leitão, " The outer membrane protein A (OmpA) and the acyl carrier protein (ACP) are virulence determinants of Burkholderia cepacia J2315", MICROBIOTEC XXXIII JPG 2007, 30.11-2.12, Lisboa, Portugal, 2007

J.H. Leitão, S.A. Sousa, M.V. Cunha, M.J. Salgado, J. Melo-Cristino, C. Barreto, I.Sá-Correia, "Antimicrobial susceptibility profiles of Burkholderia cepacia complex isolates obtained in the major Portuguese cystic fibrosis treatment center: a five-year survey", MICROBIOTEC XXXIII JPG 2007, 30.11-2.12, Lisboa, Portugal, 2007

A.S. Ferreira, J.H. Leitão, S.A. Sousa, I. Sá-Correia, L.M. Moreira, "Identification of bce cluster II of genes involved in the biosynthesis of the exopolysaccharide cepacian by bacteria of the Burkholderia genus", MICROBIOTEC XXXIII JPG 2007, 30.11-2.12, Lisboa, Portugal, 2007

C.G. Ramos, S.A. Sousa, J.H. Leitão,"Identification of a phenazine biosynthetic locus necessary for full virulence of Burkholderia cenocepacia K56-2 to the nematode Caenorhabditis elegans", MICROBIOTEC XXXIII JPG 2007, 30.11-2.12, Lisboa, Portugal, 2007

S.A. Sousa, C.G. Ramos, J.H. Leitão, "Unveiling the role of Hfq on the virulence of Burkholderia cepacia complex", RNA 2007 - IV Encontro Nacional, 8-10 November, Vimeiro, Portugal,2007

C. G. Ramos, S. A Sousa, J. Wopperer, L. Eberl, J. H. Leitão, "Isochorismatase plays a role on the virulence of Burkholderia cepacia in the nematode Caenorhabditis elegans", XVth National Congress of Biochemistry, 8-10 December, Aveiro, Portugal, 2006

S. A. Sousa, L. M. Moreira, I. Sá-Correia, J. H. Leitão, "Functional analysis of the bceA gene of the Burkholderia cepacia exopolysaccharide biosynthetic cluster", XVth National Congress of Biochemistry, 8-10 December, Aveiro, Portugal, 2006

A. S. Ferreira, J. H. Leitão, S. A. Sousa, A. M. Cosme, M. R. Santos, I. Sá-Correia, L. M. Moreira, "Importance of a tyrosine kinase and a phosphotyrosine phosphatase in cepacian biosynthesis by Burkholderia cepacia" 2nd FEMS Congress, July 4-8, Madrid, 2006

S.A. Sousa, I Sá-Correia , J. H. Leitão, "bceA encodes a type II phosphomannose isomerase involved in Cepacian biosynthesis by Burkholderia cepacia complex", 2nd FEMS Congress, July 4-8, Madrid, 2006

S.A. Sousa, J.H. Leitão, “Cloning and functional analysis of the bceA gene involved in exopolysaccharide biosynthesis by Burkholderia cepacia complex”, Microbiotec ´05, Póvoa de Varzim, 30/11-3-12, 2005

A.S. Ferreira, J.H. Leitão, I Sá-Correia, L.M. Moreira, "A tyrosine kinase and a phosphotyrosine phosphatase are involved in the biosynthesis of the exopolysaccharide by Burkholderia cepacia", Microbiotec ´05, Póvoa de Varzim, 30/11-3-12, 2005

F.S. Almeida, S.A. Sousa, M.V. Cunha, I. Sá-Correia, J.H. Leitão, “Identification of genes involved in the resistance of Burkholderia cenopacia J2315 to antimicrobials of clinical relevance”, Microbiotec ´05, Póvoa de Varzim, 30/11-3-12, 2005

S.A. Sousa, J.H. Leitão, M.V. Cunha, L.M. Moreira, G. Doring, I. Sá-Correia, “The exopolysaccharide produced by cystic fibrosis isolates of the Burkholderia cepacia complex is a virulence determinant”, Microbiotec ´05, Póvoa de Varzim, 30/11-3-12, 2005

S.A. Sousa, I. Sá-Correia, J.H. Leitão, “The bceA gene required for exopolysaccharide biosynthesis in the Burkholderia cepacia complex encodes the bifunctional phosphomannose isomerase/GDP-mannose pyrophosphorylase”, BioMicroWorld 2005, Badajoz, Spain, March 15-18, 2005

J.H. Leitão, L.M. Moreira, M.V. Cunha, S.A. Sousa, P.A. Videira, A.M. Cosme, I. Sá-Correia “Exopolysaccharide from Cystic Fibrosis-associated Burkholderia cepacia complex isolates: biosynthesis and possible involvement in pathogenesis”, BioMicroWorld 2005, Badajoz, Spain, March 15-18, 2005

L.M. Moreira, J.H. Leitão, M.V. Cunha, S.A. Sousa, P.A. Videira, A.M. Cosme, I. Sá-Correia “Exopolysaccharide biosynthesis by Cystic Fibrosis-associated isolates of the Burkholderia cepacia complex and its possible involvement in pathogenesis”, ASM Conference on Functional Genomics and Bioinformatics Approaches to Infectious Disease Research, Portland, Oregon USA, 6-9 October, 2004

D. Worlitzsch, S. Nonnenmacher, M. Ulrich, S. A. Sousa, J.H. Leitão, I. Sá-Correia, T. Coenye, P. Vandamme, G. Döring, “A rabbit antiboby specific for exopolysaccharides of 10 different Burkholderia cepacia genomovars detects B. cepacia macrocolony formation in sputum of CF patients”, 26th Congress European Cystic Fibrosis Society, Belfast, June 2003

M.V. Cunha, S. Sousa, J.H. Leitão, L.M. Moreira, P.A. Videira, I. Sá-Correia, “Respiratory infections with Burkholderia cepacia in Portuguese patients with cystic fibrosis: biosynthesis and role of the exopolysaccharide", Congresso Nacional de Bioquímica, Lisbon/Portugal, December 2002

M.V. Cunha, S. Sousa, J.A. Richau, J.H. Leitão, I. Sá-Correia, “Exopolysaccharide biosynthesis in Burkholderia cepacia isolates involved in pulmonary infections in cystic fibrosis patients”, 11th European Carbohydrate Symposium, Lisboa, 2-7 September 2001

 

Nucleotide Sequences Deposited in the GenBank:


C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-F3 16S ribosomal RNA gene, partial sequence. Access number GQ428132 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_1 16S ribosomal RNA gene, partial sequence. Access number GQ428133

 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_2 16S ribosomal RNA gene, partial sequence. Access number GQ4281324

 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_3 16S ribosomal RNA gene, partial sequence. Access number GQ428135

C.G. Ramos, S.A. Sousa, J.H. Leitão. Nucleotide sequence of Burkholderia cenocepacia strain K56-2 locus encoding the Pbr protein required for phenazine biosynthesis. Access number EU874251 (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=213959327).

S.A. Sousa, C.G. Ramos, J.H. Leitão. Nucleotide sequence of the Burkholderia cepacia IST408 hfq gene. Accession number EU760353. (release date November 2008)

S.A. Sousa, J.H. Leitão. Nucleotide sequence of the bceA gene from Burkholderia cepacia IST408. Accession number DQ463418.


L.M. Moreira, S.A. Sousa, J.H. Leitão. Nucleotide sequence of the bceD, bceE, bceF, and bceG genes from Burkholderia cepacia IST408. Accession number DQ418767