Nuno Gonçalo Pereira Mira

(Assistant Professor) *

Alternative Pages: Google Scholar; Research ID; iBB webpage; IST webpage

Status at iBB - Institute for Bioengineering and Biosciences:


  • Ph.D. in Biotechnology (supervision: I. Sá-Correia), IST, UTL, 2008

  • Graduation in Chemistry-Biochemistry Profile, IST, UTL, Lisbon, Portugal, 2004


  •  Assistant Professor, DEB, IST, University of Lisbon, 2011 - present

  • Post-Doc Researcher, IBB/CEBQ/BSRG, IST, 2008 - 2011

Research Activities:

  • Gene and genomic regulation in Saccharomyces cerevisiae, Candida glabrata and Zygosaccharomyces bailii

  • Study of molecular mechanisms underlying pathogenesis of Candida glabrata and Candida albicans

  • Epidemiology of invasive fungal infections

  • Development of molecular-based diagnosis tools for the early detection of invasive infections caused by C. glabrata and C. albicans

  • Exploitation of Saccharomyces cerevisiae as a cell factory for the production of add-value chemicals exploring systems- and synthetic biology-based approaches

Projects as PI:  

  • PANCANDIDA – Towards the development of novel diagnosis tools for the early detection of invasive infections caused by C. albicans and C. glabrata (winner project of the Gilead Génese Program)(Jan2014-Jan2016)

  • LEVITA-Towards microbial sustainable production of itaconic and levulinic acids, EraSynBio Twinning program, Apr-Oct2014


  • PhD Scolarship from "Fundação para a Ciência e a Tecnologia" (SFRH/17456/2004)

  • Post-Doc Scolarship from "Fundação para a Ciência e a Tecnologia" (SFRH/BPD/46982/2008)



2008: PhD thesis in Biotechnology “Adaptation to weak acid food preservatives in Saccharomyces cerevisiae: the Haa1 and Rim101 signalling pathways"

Edition of books

I. Sá-Correia, M.C. Teixeira, N.P. Mira, "Book of Abstracts - PYFF6 - 6th Conference on Physiology of Yeasts and Filamentous Fungi", 2016 (ISBN: 978-989-20-6736-0)

Book Chapters

N.P. Mira, M.C. Teixeira, I. Sá-Correia, "Characterization of complex regulatory networks and identification of promoter regulatory elements in yeast: in silico and wet-lab", In: Methods in Molecular Biology - Transcriptional Regulation: Methods and Protocols (Vancura A., Ed), Springer, vol. 809, 27-48, 2012.

Papers in international journals

Cardoso, J.M.S., Guerreiro, S.I., Lourenço, A., Alves, M.M., Montemor, M.F., Mira, N.P.*, Leitão, J.H.*, Carvalho, M.F.N.N.* Ag(I) camphorimine complexes with antimicrobial activity towards clinically important bacteria and species of the Candida genus. PLOS ONE, 12(5):e0177355, 2017. (*co-corresponding authors)

Henriques, S.F., Mira, N.P., Sá-Correia, I., Genome-wide search for candidate genes for yeast robustness improvement against formic acid reveals novel susceptibility (Trk1 and positive regulators) and resistance (Haa1-regulon) determinants, Biotechnology for Biofuels, in press.

Godinho, C.P., Mira, N.P., Cabrito, T.R., Teixeira, M.C., Alasoo, K., Guerreiro, J. F., Sá-Correia, I., Yeast response and tolerance to benzoic acid involves the Gcn4- and Stp1- regulated multidrug/multixenobiotic resistance transporter Tpo1, Applied Microbiology and Biotechnology, accepted for publication.

Cunha, D.*, Salazar, B.*, Lopes, M.M., Mira, N.P., Mechanistic insights underlying tolerance to acetic acid stress in vaginal Candida glabrata isolates, Frontiers in Microbiology, 8:259, 2017. (*co-first authors)

Seixas, I., Barbosa, C., Salazar, S.B., Mendes-Faia, A., Wang, Y., Guldener, U., Mendes-Ferreira, A.*, Mira, N.P.*, Genome sequence of the non-conventional yeast Hanseniaspora guilliermondii UTAD222, Genome Announcements, 5(5) e01515-16, 2017. (* co-corresponding authors)

Bernardo, R., Cunha, D.V., Wang, C., Pereira, L., Silva, S., Salazar, S.B., Schroeder, M.S., Okamoto, M., Takahashi-Nakaguchi, A., Chibana, H., Aoyoama, T., Sá-Correia, I., Azeredo, J., Butler, G., Mira, N.P., "The CgHaa1-regulon mediates response and tolerance to acetic acid stress in the human pathogen Candida glabrata", G3: Genes, Genomes and Genetics,7(1):1-18, 2017.

Santos, R., Costa, C., Mil-Homens, D., Romão, D., de Carvalho, C.C.R., Pais, P., Mira, N.P., Fialho, A.M., Teixeira, M.C.,  The multidrug resistance transporters CgTpo1_1 and CgTpo1_2 play a role in virulence and biofilm formation in the human pathogen Candida glabrata", Cellular Microbiology, doi: 10.1111/cmi.12686, in press.

Alves, M.M., Cunha, D.V., Santos, C.F., Mira, N.P., Montemor, M.F., "In vitro corrosion behaviour and anti-Candida spp. activity of Zn coated with ZnO-nanostructured ‘Anastacia’ flowers", J Mat. Chem. B, 4:4754, 2016.

Palma, P., Roque, F.C., Guerreiro, J., Mira, N.P., Queiroz, L., Sá-Correia, I., "Search for genes responsible for the remarkably high acetic acid tolerance of a Zygosaccharomyces bailii-derived interspecies hybrid strain", BMC Genomics, 16:1070, 2015.

Barbosa, C., Mendes-Faia, A., Lage, P., Mira, N.P., Mendes-Ferreira, A., "Genomic expression program of Saccharomyces cerevisiae along a mixed-culture wine fermentation with Hanseniaspora guilliermondii". Microb Cell Fact. 14(1):124, 2015.

Trček J, Mira NP, Jarboe LR. Adaptation and tolerance of bacteria against acetic acid. Appl Microbiol Biotechnol. 99(15):6215-29, 2015.

Pereira, F.B., Teixeira, M.C., Mira, N.P., Sá-Correia, I., Domingues, L., "Genome-wide screening of Saccharomyces cerevisiae genes required to foster tolerance towards industrial wheat straw hydrolysates", Journal of Industrial Microbiology & Biotechnology, 41: 1753-61, 2014.

Costa, C., Nunes, J., Henriques, A., Mira, N.P., Nakayama, H., Chibana, H., Teixeira, M.C., The Candida glabrata drug:H+ antiporter CgTpo3 (ORF CAGL0I10384g): role in azole drug resistance and polyamine homeostasis, Journal of Antimicrobial Chemotherapy, 69:1767-76, 2014.

Mira, N.P., Münsterkötter, M., Dias-Valada, F., Santos, J., Palma, M., Roque, F., Guerreiro, J., Rodrigues, F., Sousa, M.J., Leão, C., Guldener, U., Sá-Correia, I., The genome sequence of the highly acetic acid-tolerant Zygosaccharomyces bailii derived interspecies hybrid strain ISA1307, isolated from a sparkling wine plant, DNA Research, 21(3):299-313, 2014.

Rodrigues, R.M.M., de Carvalho, J., Henriques, S.F., Mira, N.P., Sá-Correia, I., Ferreira, G.N.M., "Transmission Line Model Analysis of Transcription Factors Binding to Oligoduplexes - Differentiation of the Effect of Single Nucleotide Modifications", Analyst, 139(16):3871-4, 2014.

de Carvalho, J., Rodrigues, R.M.M., Tomé, B., Henriques, S.F., Mira, N.P., Sá-Correia, I., Ferreira, G.N.M., Conformational and Mechanical changes of DNA upon transcription factor binding detected by a QCM and transmission line model, Analyst, 139(8):1847-55, 2014.

Teixeira, M.C., Monteiro, P.T., Guerreiro, J.F., Gonçalves, J.P., Mira, N.P., dos Santos, S.C., Cabrito, T., Palma, M., Costa, C., Francisco, A.P., Madeira, S.C., Oliveira, A.L., Freitas, A.T., Sá-Correia, I., "The YEASTRACT database: an upgraded information system for the analysis of gene and genomic transcription regulation in Saccharomyces cerevisiae", Nucleic Acids Research, 42: D161-D166, 2014.

Mira, N.P., Teixeira, M.C., "Microbial mechanisms of tolerance to weak acid stress", Frontiers in Microbiology (Editorial), 4, 416, 2013.

Teixeira, M.C., Godinho, C.P., Cabrito, T.R., Mira, N.P., Sá-Correia, I., Increased expression of the yeast multidrug resistance ABC transporter Pdr18 leads to increased ethanol tolerance and ethanol production in high gravity alcoholic fermentation, Microbial Cell Factories, 11, 98, 2012.

dos Santos, S.C., Mira, N.P., Moreira, A.S., Sá-Correia, I., Quantitative- and phospho-proteomic analysis of the yeast response to the tyrosine kinase inhibitor imatinib. OMICS: A Journal of Integrative Biology, 16, 537-51, 2012.

Guerreiro, J.F., Mira, N.P., Sá-Correia, I., Adaptive response to acetic acid in the highly resistant species Zygosaccharomyces bailii revealed by quantitative proteomics, PROTEOMICS, 12, 2303-18, 2012.

Pereira, F.B., Guimarães, P.M.R., Gomes, D.G., Mira, N.P., Teixeira, M.C., Sá-Correia, I., Domingues, L., Identification of candidate genes for yeast engineering to improve bioethanol production in Very-High-Gravity and lignocellulosic biomass industrial fermentations. Biotechnology for Biofuels, 4, 57, 2011.

Mira, N.P., Madeira, A., Moreira, A.S., Coutinho, A.P., Sá-Correia, I., Genomic expression analysis reveals strategies of Burkholderia cenocepacia to adapt to the airways of cystic fibrosis patients and antimicrobial therapy . PLOS One, 6, e28831, 2011.

Coutinho, C.P., dos Santos, S.C., Madeira, A., Mira, N.P., Moreira, A.S., Sá-Correia, I., Long-term colonization of the cystic fibrosis lung by Burkholderia cepacia complex bacteria: epidemiology, clonal variation and genome-wide expression alterations. Frontiers in Cellular and Infection Microbiology, 1, article12, 2011.

Gonçalves, J.P., Francisco, A.P., Mira, N.P., Teixeira, M.C., Sá-Correia, I., Oliveira, A.L., Madeira, S.C., TFRank: Network-based prioritization of regulatory associations underlying transcriptional responses. Bioinformatics, 27, 3149-57, 2011.

Mira, N.P., Henriques, S., Keller, G., Teixeira, M.C., Matos, R., Arraiano, C., Winge, D.R., Sá-Correia, I., Identification of a DNA binding site for the transcription factor Haa1p, required for Saccharomyces cerevisiae response to acetic acid stress. Nucleic Acids Research, 16, 6898-907, 2011.

Teixeira M.C., Mira N.P., Sá-Correia I., A genome-wide perspective on the response and tolerance to food-relevant stresses in Saccharomyces cerevisiae. Current Opinion in Biotechnology, 22, 150-156, 2011 (abstract).

Mira, N.P., Palma, M., Guerreiro, J., Sá-Correia I., Genome-wide identification of Saccharomyces cerevisiae genes required for tolerance to acetic acid. Microbial Cell Factories, 9:79, 2011 (abstract)

Abdulrehman D., Monteiro P.T., Teixeira M.C., Mira N.P., Lourenço A.B., dos Santos S.C., Cabrito T.R., Francisco A., Madeira S.C., Santos R., Oliveira, A.L., Sá-Correia I., Freitas A. T., “YEASTRACT: Providing a programmatic access to curated transcriptional regulatory associations in Saccharomyces cerevisiae through a web services interface.” Nucleic Acids Research, 39, D136-140, 2011

Mira N.P., Becker J.D., Sá-Correia I., Genomic expression program involving the Haa1p-regulon in Saccharomyces cerevisiae response to acetic acid. OMICS: a Journal of Integrative Biology, 14(5), 587-601, 2010 (abstract)

Mira N.P., Teixeira M.C., Sá-Correia I., Adaptive response and tolerance to weak acids in Saccharomyces cerevisiae : a genome-wide view. OMICS: a Journal of Integrative Biology, 14(5), 525-540, 2010 (abstract)

Teixeira M.C., Raposo L.R., Mira N.P., Lourenço A.B., and Sá-Correia I., "Genome-wide identification of genes required for maximal tolerance to ethanol in yeast: important role of FPS1" Applied Environmental Microbiology, 75: 5761-5772, 2009

Mira N.P., Lourenço A.,  Fernandes A., Becker J., Sa-Correia I., The RIM101 pathway has a role in Saccharomyces cerevisiae adaptive response and resistance to propionic acid and other weak acids, FEMS Yeast Research,  9, 202-216, 2009 (abstract)

Sá-Correia I., dos Santos S.C., Teixeira M.C., Cabrito T.R., Mira N.P., "Drug:H+ antiporters in chemical stress response in yeast". Trends in Microbiology, 17, 22-31, 2009 (abstract)

Monteiro P., Mendes N., Teixeira M.C., d'Orey S., Tenreiro S., Mira N.P., Pais H., Francisco A., Carvalho A., Lourenço A.R., Sá-Correia I., Oliveira A.L., Freitas A.T. "YEASTRACT-DISCOVERER: new tools to improve the analysis of transcriptional regulatory associations in Saccharomyces cerevisiae". Nucleic Acids Res. Database Issue 36: D132-D136, 2008 (abstract)

Simões T., Mira N.P., Fernandes A.R., Sá-Correia I., "The SPI1 gene, encoding a glycosylphosphatidylinositol (GPI)-anchored cell wall protein, plays a prominent role in the development of yeast resistance to lipophilic weak acids food preservatives", Applied and Environmental Microbiology, 72, 7168-7175, 2006 (abstract)

Teixeira M.C., Fernandes A.R., Mira N.P., Becker J.D., Sá-Correia I., "Early transcriptional response of Saccharomyces cerevisiae to stress imposed by the herbicide 2,4-dichlorophenoxyacetic acid", FEMS Yeast Research, 6, 230–248, 2006 (abstract)

Teixeira M.C., Monteiro P., Jain P., Tenreiro S., Fernandes A.R., Mira N.P., Alenquer M., Freitas A.T., Oliveira A.L., Sá-Correia I., "The YEASTRACT database: a tool for the analysis of transcriptional regulatory associations in Saccharomyces cerevisiae", Nucleic Acids Research, Database Issue, 34, D446-D451, 2006 (abstract)

Fernandes A.R., Mira N.P., Vargas R.C., Canelhas I., Sá-Correia I., “Saccharomyces cerevisiae adaptation to weak acids involves the transcription factor Haa1p and of Haa1p-regulated genes”, Biochemical and Biophysical Research Communications, 337, 95-103, 2005 (abstract)

Papers in national journals with referees:

Teixeira M. C., Monteiro P., Jain P., Tenreiro S., Fernandes A. R., Mira N. P., Alenquer M., Freitas A. T., Oliveira  A.L., Sá-Correia I., "A Bioinformática e as Bases de Dados: o exemplo de uma Base de Dados para Análise de Mecanismos de Regulação em Levedura", Boletim de Biotecnologia, 81, 23-31,  2005.