André Manuel Sardinha Grilo *

Alternative Pages: Google Scholar; Research ID; iBB webpage

Status at iBB - Institute for Bioengineering and Biosciences:

·         Researcher of the Biological Sciences Research Group

Education:

  • Graduation in Applied Microbiology, Faculty of Sciences, University of Lisboa, Portugal, 2009

Positions:

  • PhD student in Biotechnology under the supervision of Professor Jorge H. Leitão

  • Degree in Microbiology, joint degree from the Faculty of Sciences and Faculty of Medicine, University of Lisboa, 2008

Research Activities:

  • Molecular characterization of microbial populations from UASB reactors;

  • Roles of RNA chaperones and small non-coding RNAs in the biology and virulence of bacteria of the Burkholderia cepacia complex;

Grants:

  • PhD Scolarship from Fundação para a Ciência e Tecnologia (SFRH/BD/70696/2010)

  • Research grant from the FCT Project PTDC/AMB/65025/2006 (May 2009-November 2010).

Publications:

Thesis

Grilo, A.M., Caracterização molecular de populações microbianas em reactores anaeróbios, Faculty of Sciences of the Lisbon University, 2009 (Supervisor: Jorge H. Leitão)

Books

C.G. Ramos, A.M Grilo, J.H. Leitão. Small non-coding RNAs in Prokaryotes: Roles in Pathogenesis and Potential Therapeutic Targets. Lambert Academic Publishing, Saarbrücken, Germany, 2010 (ISBN: 978-3-8383-2116-5)

 

Book chapters

 

C.G. Ramos, Grilo A.M., da Costa P.J.P., Nadais H., Leitão J.H., "Extraction and Purification of DNA obtained from UASB reactors sludge samples". In: DNA extraction: Methods, Applications and Limitations. Nova Science Publishers, Inc. (Accepted for publication).

Papers in International Journals

Feliciano, J.R., Grilo, A.M., Guerreiro, S.I., Sousa, S.A., Leitão, J.H., "Hfq: a multifaceted RNA chaperone involved in virulence", Future Microbiology, accepted for publication.

Ramos, C.G., Grilo, A.M., Sousa, S.A., Feliciano, J.R., da Costa, P.J., Leitão, J.H., "Regulation of Hfq mRNA and protein levels in Escherichia coli and Pseudomonas aeruginosa by the Burkholderia cebocepacia MtvR sRNA", PLoS One, 9(6): e98813, 2014.

Sousa, S.A., Feliciano, J.R., Grilo, A.M., Leitão, J.H., "Bioinformatics: a molecular microbiologist's perspective", Current Bioinformatics, 9: 8-17, 2014.

Ramos, C.G., Grilo, A.M., da Costa, P.J., Leitão, J.H., "Experimental identification of small non-coding regulatory RNAs in the opportunistic human pathogen Burkholderia cenocepacia", Genomics. 101: 139-148, 2013.

H. Nadais, M. Barbosa, I. Capela, L. Arroja, C.G. Ramos, A. Grilo, S.A. Sousa, J.H. Leitão, Enhancing Wastewater Degradation and Biogas Production by Intermittent Operation of UASB Reactors. Energy. 36: 2146-68, 2011.

C.G. Ramos, S.ASousa, A.M. Grilo, L. Eberl, J.H. Leitão, The Burkholderia cenocepacia K56-2 pleiotropic regulator Pbr, is required for stress resistance and virulence. Microbial Pathogenesis. 48: 168-177, 2010.

C.G. Ramos, A.M. Grilo, S.A. Sousa, M.L. Barbosa, H. Nadais, J.H. Leitão,  A new methodology combining PCR, cloning, and sequencing of clones  discriminated by RFLP for the study of microbial populations:  application to an UASB reactor sample. Applied Microbiology and  Biotechnology 85:801-806, 2010.

Papers in National Journals

A.M. Grilo, C.G. Ramos, S.A. Sousa, M.L. Barbosa, H. Nadais, J.H. Leitão. Caracterização molecular de populações microbianas em reactores anaeróbios para o tratamento de efluentes, Boletim de Biotecnologia, 85:3-9, 2010.

Papers in Conference Proceedings

Ramos, C.G., Sousa, S.A., Grilo, A.M., da Costa, P.J.P., Feliciano, J.R, Leitão, J.H., A RNomics-based strategy identifies regulatory small RNAs in Burkholderia cenocepacia complex. Proceedings of the First Portuguese Meeting in Bioengineering – IEEE-EMBS, 1-4 March 2011, IST, TagusPark, Oeiras, Portugal

 

M. Barbosa, C. G. Ramos, A. M. Grilo, S. A. Sousa, I. Capela, L. Arroja, J. H. Leitão, H. Nadais, Bioenergy production from complex wastewaters, Proceedings of the 5th International Conference on ENERGY, ENVIRONMENT, ECOSYSTEMS and SUSTAINABLE DEVELOPMENT (EEESD '09), Vouliagmeni, Athens, Greece, September 28-30, 2009. Energy and Environmental Engineering Series, Published by WSEAS Press. ISSN: 1790-5095, ISBN: 978-960-474-125-0

 

Abstracts in national and international meetings:

Oral Communications

Ramos, C.G., Sousa, S.A., Grilo, A.M., da Costa, P.J.P., Feliciano, J.R, Leitão, J.H., A RNomics-based strategy identifies regulatory small RNAs in Burkholderia cenocepacia complex. First Portuguese Meeting in Bioengineering – IEEE-EMBS, 1-4 March 2011, IST, TagusPark, Oeiras, Portugal

 

Written Communications (Poster)

Ramos, C.G., Sousa,S.A., Feliciano, J.R., Grilo, A.M., Leitão, J.H., Functional analysis of the Burkholderia cenocepacia J2315 hfq2 gene encoding an unsual RNA chaperone, and identification of the small non-coding RNA LhdR, The Non Coding Genome EMBO-EMBL Symposium 2010, 13-16 October , Heidelberg, Germany

C. G. Ramos, S. A. Sousa, A. M. Grilo, J. H. Leitão, The Pbr transcription regulator of Burkholderia cenocepacia K56-2 is required for phenazine production and virulence, 3rd Congress of European Microbiologists (FEMS), June 28 – July 3 2009, Gotenborg, Sweden.

C.G. Ramos, A.M. Grilo, S.A. Sousa, I. Capela, L. Arroja, J.H. Leitão and H. Nadais, Characterization of microbial populations developed in UASB reactors operated in continuous and intermittent mode, ASPD5 conference- 24th -27th of May, 2009, Aalborg, Denmark

S.A. Sousa, C. G. Ramos, H. Nadais, J. H. Leitão, Molecular characterization of microbial populations in sludge bed reactors for dairy industry wastewater treatment, XVIth National Congress of Biochemistry, Azores, Portugal, 22-25 October 2008.

Nucleotide Sequences Deposited in the GenBank

 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-F3 16S ribosomal RNA gene, partial sequence. Access number GQ428132

 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_1 16S ribosomal RNA gene, partial sequence. Access number GQ428133

 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_2 16S ribosomal RNA gene, partial sequence. Access number GQ4281324

 

C.G. Ramos, A.M. Grilo, S.A. Sousa, H. Nadais and J.H. Leitão, Dairy wastewater treatment UASB reactor uncultured bacterium clone IST-C_3 16S ribosomal RNA gene, partial sequence. Access number GQ428135